ARG20932

anti-CD61 / Integrin beta 3 antibody [PM6/13] (FITC)

anti-CD61 / Integrin beta 3 antibody [PM6/13] (FITC) for Flow cytometry,ICC/IF,Western blot,Blocking,ELISA and Human

Overview

Product Description FITC-conjugated Mouse Monoclonal antibody [PM6/13] recognizes CD61 / Integrin beta 3
Tested Reactivity Hu
Tested Application BL, ELISA, FACS, ICC/IF, WB
Specificity Human CD61.
Host Mouse
Clonality Monoclonal
Clone PM6/13
Isotype IgG1, kappa
Target Name CD61 / Integrin beta 3
Antigen Species Human
Immunogen Human platelet plasma membrane
Conjugation FITC
Full Name integrin, beta 3 (platelet glycoprotein IIIa, antigen CD61)
Alternate Names GT; CD antigen CD61; CD61; BDPLT2; GPIIIa; BDPLT16; GP3A; Platelet membrane glycoprotein IIIa; Integrin beta-3

Application Instructions

Application Suggestion
Tested Application Dilution
BLAssay-dependent
ELISAAssay-dependent
FACS10 µl/10^6 cells
ICC/IFAssay-dependent
WBAssay-dependent
Application Note WB: Under non-reducing condition.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Calculated MW 87 kDa

Properties

Form Liquid
Buffer PBS and Sodium azide
Preservative Sodium azide
Storage instruction Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database links

GeneID: 3690 Human ITGB3

Swiss-port # P05106 Human Integrin beta-3

Gene Symbol ITGB3
Gene Full Name integrin, beta 3 (platelet glycoprotein IIIa, antigen CD61)
Background The ITGB3 protein product is the integrin beta chain beta 3. Integrins are integral cell-surface proteins composed of an alpha chain and a beta chain. A given chain may combine with multiple partners resulting in different integrins. Integrin beta 3 is found along with the alpha IIb chain in platelets. Integrins are known to participate in cell adhesion as well as cell-surface mediated signalling. [provided by RefSeq, Jul 2008]
Function Integrin alpha-V/beta-3 (ITGAV:ITGB3) is a receptor for cytotactin, fibronectin, laminin, matrix metalloproteinase-2, osteopontin, osteomodulin, prothrombin, thrombospondin, vitronectin and von Willebrand factor. Integrin alpha-IIb/beta-3 (ITGA2B:ITGB3) is a receptor for fibronectin, fibrinogen, plasminogen, prothrombin, thrombospondin and vitronectin. Integrins alpha-IIb/beta-3 and alpha-V/beta-3 recognize the sequence R-G-D in a wide array of ligands. Integrin alpha-IIb/beta-3 recognizes the sequence H-H-L-G-G-G-A-K-Q-A-G-D-V in fibrinogen gamma chain. Following activation integrin alpha-IIb/beta-3 brings about platelet/platelet interaction through binding of soluble fibrinogen. This step leads to rapid platelet aggregation which physically plugs ruptured endothelial surface. Fibrinogen binding enhances SELP expression in activated platelets (By similarity). In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions. [UniProt]
PTM Phosphorylated on tyrosine residues in response to thrombin-induced platelet aggregation. Probably involved in outside-in signaling. A peptide (AA 740-762) is capable of binding GRB2 only when both Tyr-773 and Tyr-785 are phosphorylated. Phosphorylation of Thr-779 inhibits SHC binding.