anti-CD71 / Transferrin Receptor antibody [RI7217]
anti-CD71 / Transferrin Receptor antibody [RI7217] for Flow cytometry,ICC/IF,Immunoprecipitation,Western blot,Blocking and Mouse
|Product Description||Rat Monoclonal antibody [RI7217] recognizes CD71 / Transferrin Receptor|
|Tested Application||BL, FACS, ICC/IF, IP, WB|
|Specificity||Mouse CD71. The clone RI7217 inhibits cell proliferation in vitro.|
|Target Name||CD71 / Transferrin Receptor|
|Immunogen||DMSO induced Friend erythroleukemia 745.6|
|Full Name||transferrin receptor|
|Alternate Names||TFR1; CD antigen CD71; CD71; T9; p90; TR; Trfr; Transferrin receptor protein 1; TRFR; sTfR; TfR1; TfR; TFR|
|Application Note||* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.|
|Calculated MW||85 kDa|
|Buffer||BBS (pH 8.2)|
|Storage instruction||For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.|
|Note||For laboratory research only, not for drug, diagnostic or other use.|
|Gene Full Name||transferrin receptor|
|Background||This gene encodes a cell surface receptor necessary for cellular iron uptake by the process of receptor-mediated endocytosis. This receptor is required for erythropoiesis and neurologic development. Multiple alternatively spliced variants have been identified. [provided by RefSeq, Sep 2015]|
|Function||Cellular uptake of iron occurs via receptor-mediated endocytosis of ligand-occupied transferrin receptor into specialized endosomes. Endosomal acidification leads to iron release. The apotransferrin-receptor complex is then recycled to the cell surface with a return to neutral pH and the concomitant loss of affinity of apotransferrin for its receptor. Transferrin receptor is necessary for development of erythrocytes and the nervous system (By similarity). A second ligand, the heditary hemochromatosis protein HFE, competes for binding with transferrin for an overlapping C-terminal binding site. [UniProt]|
|PTM||N- and O-glycosylated, phosphorylated and palmitoylated. The serum form is only glycosylated.
Proteolytically cleaved on Arg-100 to produce the soluble serum form (sTfR).
Palmitoylated on both Cys-62 and Cys-67. Cys-62 seems to be the major site of palmitoylation.