ARG20945

anti-CD71 / Transferrin Receptor antibody [RI7217] (PE)

anti-CD71 / Transferrin Receptor antibody [RI7217] (PE) for Flow cytometry,ICC/IF,Western blot,Blocking and Mouse

Overview

Product Description PE-conjugated Rat Monoclonal antibody [RI7217] recognizes CD71 / Transferrin Receptor
Tested Reactivity Ms
Tested Application BL, FACS, ICC/IF, WB
Specificity Mouse CD71. The clone RI7217 inhibits cell proliferation in vitro.
Host Rat
Clonality Monoclonal
Clone RI7217
Isotype IgG2a, kappa
Target Name CD71 / Transferrin Receptor
Species Mouse
Immunogen DMSO induced Friend erythroleukemia 745.6
Conjugation PE
Full Name transferrin receptor
Alternate Names TFR1; CD antigen CD71; CD71; T9; p90; TR; Trfr; Transferrin receptor protein 1; TRFR; sTfR; TfR1; TfR; TFR

Application Instructions

Application Suggestion
Tested Application Dilution
BLAssay-dependent
FACS< 0.2 µg/10^6 cells
ICC/IFAssay-dependent
WBAssay-dependent
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Calculated MW 85 kDa

Properties

Form Liquid
Buffer PBS, Sodium azide and a stabilizing agent
Preservative Sodium azide
Concentration 0.1 mg/ml
Storage instruction Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database links

GeneID: 22042 Mouse TFRC

Swiss-port # Q62351 Mouse Transferrin receptor protein 1

Gene Symbol TFRC
Gene Full Name transferrin receptor
Background This gene encodes a cell surface receptor necessary for cellular iron uptake by the process of receptor-mediated endocytosis. This receptor is required for erythropoiesis and neurologic development. Multiple alternatively spliced variants have been identified. [provided by RefSeq, Sep 2015]
Function Cellular uptake of iron occurs via receptor-mediated endocytosis of ligand-occupied transferrin receptor into specialized endosomes. Endosomal acidification leads to iron release. The apotransferrin-receptor complex is then recycled to the cell surface with a return to neutral pH and the concomitant loss of affinity of apotransferrin for its receptor. Transferrin receptor is necessary for development of erythrocytes and the nervous system (By similarity). A second ligand, the heditary hemochromatosis protein HFE, competes for binding with transferrin for an overlapping C-terminal binding site. [UniProt]
PTM N- and O-glycosylated, phosphorylated and palmitoylated. The serum form is only glycosylated.
Proteolytically cleaved on Arg-100 to produce the soluble serum form (sTfR).
Palmitoylated on both Cys-62 and Cys-67. Cys-62 seems to be the major site of palmitoylation.

Images (1) Click the Picture to Zoom In

  • ARG20945 anti-CD71 / Transferrin Receptor antibody [RI7217] (PE) FACS image

    Flow Cytometry: Mouse pre-B cell line 18-81 stained with ARG20945 anti-CD71 / Transferrin Receptor antibody [RI7217] (PE).