ARG10510

anti-CD71 / Transferrin Receptor (soluble) antibody [13E4]

anti-CD71 / Transferrin Receptor (soluble) antibody [13E4] for ELISA,Western blot and Human

Cancer antibody; Cell Biology and Cellular Response antibody; Developmental Biology antibody; Immune System antibody; Metabolism antibody

Overview

Product Description Mouse Monoclonal antibody [13E4] recognizes CD71 / Transferrin Receptor (soluble)
Tested Reactivity Hu
Tested Application ELISA, WB
Specificity Human soluble transferrin receptor and complex between soluble transferrin receptor and transferrin
Host Mouse
Clonality Monoclonal
Clone 13E4
Isotype IgG2a
Target Name CD71 / Transferrin Receptor (soluble)
Antigen Species Human
Immunogen Balb/c mice immunized with purified human soluble transferrin receptor.
Conjugation Un-conjugated
Full Name transferrin receptor
Alternate Names TFR1; CD antigen CD71; CD71; T9; p90; TR; Trfr; Transferrin receptor protein 1; TRFR; sTfR; TfR1; TfR; TFR

Application Instructions

Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Protein A affinity purified.
Buffer PBS (pH.2)) and 0.1 % Sodium azide
Preservative 0.1 % Sodium azide
Concentration 1.0-2.0 mg/ml
Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database links

GeneID: 7037 Human TFRC

Swiss-port # P02786 Human Transferrin receptor protein 1

Gene Symbol TFRC
Gene Full Name transferrin receptor
Function Cellular uptake of iron occurs via receptor-mediated endocytosis of ligand-occupied transferrin receptor into specialized endosomes. Endosomal acidification leads to iron release. The apotransferrin-receptor complex is then recycled to the cell surface with a return to neutral pH and the concomitant loss of affinity of apotransferrin for its receptor. Transferrin receptor is necessary for development of erythrocytes and the nervous system (By similarity). A second ligand, the heditary hemochromatosis protein HFE, competes for binding with transferrin for an overlapping C-terminal binding site. [UniProt]
Research Area Cancer antibody; Cell Biology and Cellular Response antibody; Developmental Biology antibody; Immune System antibody; Metabolism antibody
PTM N- and O-glycosylated, phosphorylated and palmitoylated. The serum form is only glycosylated.
Proteolytically cleaved on Arg-100 to produce the soluble serum form (sTfR).
Palmitoylated on both Cys-62 and Cys-67. Cys-62 seems to be the major site of palmitoylation.

Clone References

A human blood-brain barrier transcytosis assay reveals antibody transcytosis influenced by pH-dependent receptor binding.

Sade H et al.
PLoS One.,  (2014)

publication_link

 

hr_line

TBC1D14 regulates autophagosome formation via Rab11- and ULK1-positive recycling endosomes.

Longatti A et al.
J Cell Biol.,  (2012)

publication_link

 

hr_line