ARG56944
anti-CD73 antibody [2B6]
anti-CD73 antibody [2B6] for Flow cytometry,Western blot and Human
Overview
| Product Description | Mouse Monoclonal antibody [2B6] recognizes CD73 |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | FACS, WB |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | 2B6 |
| Isotype | IgG2b, kappa |
| Target Name | CD73 |
| Antigen Species | Human |
| Immunogen | Recombinant fragment around aa. 27-252 of Human CD73. |
| Conjugation | Un-conjugated |
| Alternate Names | 5'-nucleotidase; CD antigen CD73; eN; CALJA; CD73; EC 3.1.3.5; NT; Ecto-5'-nucleotidase; NT5; E5NT; 5'-NT; eNT; NTE |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein G. |
| Buffer | PBS (pH 7.4), 0.02% Sodium azide and 10% Glycerol. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 10% Glycerol |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | NT5E |
| Gene Full Name | 5'-nucleotidase, ecto (CD73) |
| Background | The protein encoded by this gene is a plasma membrane protein that catalyzes the conversion of extracellular nucleotides to membrane-permeable nucleosides. The encoded protein is used as a determinant of lymphocyte differentiation. Defects in this gene can lead to the calcification of joints and arteries. Two transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Mar 2011] |
| Function | Hydrolyzes extracellular nucleotides into membrane permeable nucleosides. Exhibits AMP-, NAD-, and NMN-nucleosidase activities. [UniProt] |
| Calculated MW | 63 kDa |
Images (3) Click the Picture to Zoom In
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ARG56944 anti-CD73 antibody [2B6] FACS image
Flow Cytometry: PBMC cell line stained with ARG56944 anti-CD73 antibody [2B6] at 2-5 µg for 1x10^6 cells (red line). Secondary antibody: Goat anti-Mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was Mouse IgG (black line).
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ARG56944 anti-CD73 antibody [2B6] WB image
Western blot: 30 µg of Human mesenchymal stem cell lysate stained with ARG56944 anti-CD73 antibody [2B6] at 1:1000.
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ARG56944 anti-CD73 antibody [2B6] FACS image
Flow Cytometry: LNCaP cell line stained with ARG56944 anti-CD73 antibody [2B6] at 2-5 µg for 1x10^6 cells (red line). Secondary antibody: Goat anti-Mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was Mouse IgG (black line ).
