anti-CD79a antibody [SQab1743]
anti-CD79a antibody [SQab1743] for Flow cytometry,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
|Product Description||Recombinant Rabbit Monoclonal antibody [SQab1743] recognizes CD79a|
|Tested Application||FACS, IHC-P, WB|
|Immunogen||Synthetic peptide around the N-terminus of Human CD79a.|
|Alternate Names||Surface IgM-associated protein; B-cell antigen receptor complex-associated protein alpha chain; Membrane-bound immunoglobulin-associated protein; Ig-alpha; MB-1 membrane glycoprotein; MB-1; IGA; CD antigen CD79a|
|Application Note||IHC-P: Antigen Retrieval: Boil tissue section in Tris/EDTA buffer (pH 9.0).
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
|Purification||Purification with Protein A.|
|Buffer||PBS, 0.01% Sodium azide, 40% Glycerol and 0.05% BSA.|
|Preservative||0.01% Sodium azide|
|Stabilizer||40% Glycerol and 0.05% BSA|
|Storage Instruction||For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.|
|Note||For laboratory research only, not for drug, diagnostic or other use.|
|Gene Full Name||CD79a molecule, immunoglobulin-associated alpha|
|Background||CD79a: The B lymphocyte antigen receptor is a multimeric complex that includes the antigen-specific component, surface immunoglobulin (Ig). Surface Ig non-covalently associates with two other proteins, Ig-alpha and Ig-beta, which are necessary for expression and function of the B-cell antigen receptor. This gene encodes the Ig-alpha protein of the B-cell antigen component. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Jul 2008]|
|Function||CD79a is required in cooperation with CD79b for initiation of the signal transduction cascade activated by binding of antigen to the B-cell antigen receptor complex (BCR) which leads to internalization of the complex, trafficking to late endosomes and antigen presentation. Also required for BCR surface expression and for efficient differentiation of pro- and pre-B-cells. Stimulates SYK autophosphorylation and activation. Binds to BLNK, bringing BLNK into proximity with SYK and allowing SYK to phosphorylate BLNK. Also interacts with and increases activity of some Src-family tyrosine kinases. Represses BCR signaling during development of immature B-cells. [UniProt]|
CD79a antibodies; Anti-Rabbit IgG secondary antibodies;
Tumor-Infiltrating Lymphocytes (TILs)
|Calculated MW||25 kDa|
|PTM||Phosphorylated on tyrosine, serine and threonine residues upon B-cell activation. Phosphorylation of tyrosine residues by Src-family kinases is an early and essential feature of the BCR signaling cascade. The phosphorylated tyrosines serve as docking sites for SH2-domain containing kinases, leading to their activation which in turn leads to phosphorylation of downstream targets. Phosphorylated by LYN. Phosphorylation of serine and threonine residues may prevent subsequent tyrosine phosphorylation.
Arginine methylation in the ITAM domain may interfere with the binding of SYK. It promotes signals leading to B-cell differentiation (By similarity). [UniProt]
Images (3) Click the Picture to Zoom In
ARG66254 anti-CD79a antibody [SQab1743] FACS image
Flow Cytometry: Ramos cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% TritonX-100 for 15 min. The cells were then stained with ARG66254 anti-CD79a antibody [SQab1743] (red) at 1:2000 dilution in 1x PBS/1% BSA for 30 min at room temperture, followed by Alexa Fluor® 488 labelled secondary antibody. Unlabelled sample (black) was used as a control.
ARG66254 anti-CD79a antibody [SQab1743] IHC-P image
Immunohistochemistry: Formalin-fixed and paraffin-embedded Human Tonsil tissue stained with ARG66254 anti-CD79a antibody [SQab1743] at 1:12800. Antigen Retrieval: Boil tissue section in Tris/EDTA buffer (pH 9.0).
ARG66254 anti-CD79a antibody [SQab1743] WB image
Western blot: 10 µg of Raji, Ramos and Daudi cell lysates stained with ARG66254 anti-CD79a antibody [SQab1743] at 1:1000 dilution.