ARG20956

anti-CD80 antibody [1G10] (PE-Cyanine 5)

anti-CD80 antibody [1G10] (PE-Cyanine 5) for Blocking,Flow cytometry and Mouse

Overview

Product Description PE-Cyanine 5-conjugated Rat Monoclonal antibody [1G10] recognizes CD80
Tested Reactivity Ms
Tested Application BL, FACS
Specificity Mouse CD80. The clone 1G10 blocks binding of CTLA-4 to CD80 but it does not block stimulation of T cells by natural antigen-presenting cells.
Host Rat
Clonality Monoclonal
Clone 1G10
Isotype IgG2a, kappa
Target Name CD80
Species Mouse
Immunogen Activated 5C2 cells
Conjugation PE-Cyanine 5
Full Name CD80 antigen
Alternate Names B7.1; CTLA-4 counter-receptor B7.1; CD28LG; T-lymphocyte activation antigen CD80; B7-1; CD28LG1; B7; LAB7; Activation B7-1 antigen; CD antigen CD80; BB1

Application Instructions

Application Suggestion
Tested Application Dilution
BLAssay-dependent
FACS< 0.2 µg/10^6 cells
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Calculated MW 33 kDa

Properties

Form Liquid
Buffer PBS, Sodium azide and a stabilizing agent
Preservative Sodium azide
Concentration 0.1 mg/ml
Storage instruction Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database links

GeneID: 12519 Mouse CD80

Swiss-port # Q00609 Mouse T-lymphocyte activation antigen CD80

Gene Symbol CD80
Gene Full Name CD80 antigen
Background The protein encoded by this gene is a membrane receptor that is activated by the binding of CD28 or CTLA-4. The activated protein induces T-cell proliferation and cytokine production. This protein can act as a receptor for adenovirus subgroup B and may play a role in lupus neuropathy. [provided by RefSeq, Aug 2011]
Function Involved in the costimulatory signal essential for T-lymphocyte activation. T-cell proliferation and cytokine production is induced by the binding of CD28, binding to CTLA-4 has opposite effects and inhibits T-cell activation. [UniProt]

Images (1) Click the Picture to Zoom In

  • ARG20956 anti-CD80 antibody [1G10] (PE-Cyanine 5) FACS image

    Flow Cytometry: DBA/2 Mouse mastocytoma cell line P815 (negative control) and mCD80-Ig transfected P815 cells stained with ARG20956 anti-CD80 antibody [1G10] (PE-Cyanine 5).