ARG21088

anti-CD86 antibody [2D10]

anti-CD86 antibody [2D10] for Flow cytometry,IHC-Frozen sections,Immunoprecipitation,Blocking,ELISA and Mouse

Overview

Product Description Rat Monoclonal antibody [2D10] recognizes CD86
Tested Reactivity Ms
Tested Application BL, ELISA, FACS, IHC-Fr, IP
Specificity Mouse CD86.
Host Rat
Clonality Monoclonal
Clone 2D10
Isotype IgG2b, kappa
Target Name CD86
Species Mouse
Immunogen Mouse B cell lymphoma cell line 5C2
Conjugation Un-conjugated
Full Name CD86 antigen
Alternate Names B70; B7.2; LAB72; CD antigen CD86; B7-2; FUN-1; CD28LG2; T-lymphocyte activation antigen CD86; CTLA-4 counter-receptor B7.2; Activation B7-2 antigen; BU63

Application Instructions

Application Suggestion
Tested Application Dilution
BLAssay-dependent
ELISAAssay-dependent
FACSAssay-dependent
IHC-FrAssay-dependent
IPAssay-dependent
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Calculated MW 38 kDa

Properties

Form Liquid
Buffer BBS (pH 8.2)
Concentration 0.5 mg/ml
Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database links

GeneID: 12524 Mouse CD86

Swiss-port # P42082 Mouse T-lymphocyte activation antigen CD86

Gene Symbol CD86
Gene Full Name CD86 antigen
Background This gene encodes a type I membrane protein that is a member of the immunoglobulin superfamily. This protein is expressed by antigen-presenting cells, and it is the ligand for two proteins at the cell surface of T cells, CD28 antigen and cytotoxic T-lymphocyte-associated protein 4. Binding of this protein with CD28 antigen is a costimulatory signal for activation of the T-cell. Binding of this protein with cytotoxic T-lymphocyte-associated protein 4 negatively regulates T-cell activation and diminishes the immune response. Alternative splicing results in several transcript variants encoding different isoforms.[provided by RefSeq, May 2011]
Function Receptor involved in the costimulatory signal essential for T-lymphocyte proliferation and interleukin-2 production, by binding CD28 or CTLA-4. May play a critical role in the early events of T-cell activation and costimulation of naive T-cells, such as deciding between immunity and anergy that is made by T-cells within 24 hours after activation. Isoform 2 interferes with the formation of CD86 clusters, and thus acts as a negative regulator of T-cell activation. [UniProt]
PTM Polyubiquitinated; which is promoted by MARCH8 and results in endocytosis and lysosomal degradation.