anti-CD86 antibody [BU63] (PE)

anti-CD86 antibody [BU63] (PE) for Flow cytometry and Human

Developmental Biology antibody; Immune System antibody; Microbiology and Infectious Disease antibody


Product Description

PE-conjugated Mouse Monoclonal antibody [BU63] recognizes CD86

Tested Reactivity Hu
Tested Application FACS
Specificity The clone BU63 reacts with CD86 (B7-2), a 70 kDa type I transmembrane glycoprotein of immunoglobulin supergene family, expressed on professional antigen-presenting cells, such as dendritic cells, macrophages or activated B lymphocytes.
HLDA V; WS Code BP BP072
HLDA V; WS Code A A109
HLDA VI; WS Code BP 95
HLDA VI; WS Code B CD86.9
Host Mouse
Clonality Monoclonal
Clone BU63
Isotype IgG1
Target Name CD86
Immunogen B-lymphoblastoid cell line ARH 77
Conjugation PE
Alternate Names B70; B7.2; LAB72; CD antigen CD86; B7-2; FUN-1; CD28LG2; T-lymphocyte activation antigen CD86; CTLA-4 counter-receptor B7.2; Activation B7-2 antigen; BU63

Application Instructions

Application Suggestion
Tested Application Dilution
FACS20 µl / 10^6 cells
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.


Form Liquid
Purification Note The purified antibody is conjugated with R-Phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary.
Buffer PBS, 15 mM Sodium azide and 0.2% (w/v) high-grade protease free BSA
Preservative 15 mM Sodium azide
Stabilizer 0.2% (w/v) high-grade protease free BSA
Storage Instruction Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.


Database Links

GeneID: 942 Human CD86

Swiss-port # P42081 Human T-lymphocyte activation antigen CD86

Gene Symbol CD86
Gene Full Name CD86 molecule
Background CD80 (B7-1) and CD86 (B7-2) are ligands of T cell critical costimulatory molecule CD28 and of an inhibitory receptor CTLA-4 (CD152). The both B7 molecules are expressed on professional antigen-presenting cells and are essential for T cell activation, the both molecules can also substitute for each other in this process. The question what are the differences in CD80 and CD86 competency has not been fully elucidated yet; there are still conflicts in results about their respective roles in initiation or sustaining of the T cell immune response.
Function Receptor involved in the costimulatory signal essential for T-lymphocyte proliferation and interleukin-2 production, by binding CD28 or CTLA-4. May play a critical role in the early events of T-cell activation and costimulation of naive T-cells, such as deciding between immunity and anergy that is made by T-cells within 24 hours after activation. Isoform 2 interferes with the formation of CD86 clusters, and thus acts as a negative regulator of T-cell activation. [UniProt]
Research Area Developmental Biology antibody; Immune System antibody; Microbiology and Infectious Disease antibody
Calculated MW 38 kDa
PTM Polyubiquitinated; which is promoted by MARCH8 and results in endocytosis and lysosomal degradation.

Images (1) Click the Picture to Zoom In

  • ARG53908 anti-CD86 antibody [BU63] (PE) FACS image

    Flow Cytometry: Human peripheral blood cells stained with ARG53908 anti-CD86 antibody [BU63] (PE).

Clone References

Insertion of host-derived costimulatory molecules CD80 (B7.1) and CD86 (B7.2) into human immunodeficiency virus type 1 affects the virus life cycle.

Giguère JF et al.
J Virol.,  (2004)




Antigen-presenting T cells induce the development of cytotoxic CD4+ T cells. I. Involvement of the CD80-CD28 adhesion molecules.

Mauri D et al.
J Immunol.,  (1995)




B70/B7-2 is identical to CD86 and is the major functional ligand for CD28 expressed on human dendritic cells.

Caux C et al.
J Exp Med.,  (1994)




The B7-2 (B70) costimulatory molecule expressed by monocytes and activated B lymphocytes is the CD86 differentiation antigen.

Engel P et al.
Blood.,  (1994)