anti-CD98 antibody [MEM-108] (PE)

anti-CD98 antibody [MEM-108] (PE) for Flow cytometry and Human

Developmental Biology antibody; Immune System antibody; Metabolism antibody; Signaling Transduction antibody


Product Description

PE-conjugated Mouse Monoclonal antibody [MEM-108] recognizes CD98

Tested Reactivity Hu
Tested Application FACS
Specificity The clone MEM-108 reacts with CD98, a 125 kDa disulfide-linked heterodimer (80 kDa glycosylated heavy chain + 45 kDa non-glykosylated light chain). CD98 is expressed on T lymphocytes (upon activation) and activated NK cells; it is also present at low levels on B lymphocytes, NK cells, monocytes and platelets.
HLDA VI; WS Code BP 409
HLDA VI; WS Code NL N-L017
Host Mouse
Clonality Monoclonal
Clone MEM-108
Isotype IgG1
Target Name CD98
Antigen Species Human
Immunogen RAJI human Burkitt's lymphoma cell line
Conjugation PE
Alternate Names MDU1; 4T2HC; CD98; 4F2hc; 4F2 cell-surface antigen heavy chain; CD98HC; 4F2HC; NACAE; Solute carrier family 3 member 2; CD antigen CD98; 4F2 heavy chain antigen; Lymphocyte activation antigen 4F2 large subunit; 4F2

Application Instructions

Application Suggestion
Tested Application Dilution
FACS20 µl / 10^6 cells
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.


Form Liquid
Purification Note The purified antibody is conjugated with R-Phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary.
Buffer PBS, 15 mM Sodium azide and 0.2% (w/v) high-grade protease free BSA
Preservative 15 mM Sodium azide
Stabilizer 0.2% (w/v) high-grade protease free BSA
Storage Instruction Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.


Database Links

GeneID: 6520 Human SLC3A2

Swiss-port # P08195 Human 4F2 cell-surface antigen heavy chain

Gene Symbol SLC3A2
Gene Full Name solute carrier family 3 (amino acid transporter heavy chain), member 2
Background CD98 (4F2) is a type II transmembrane glycoprotein which serves as the heavy chain of the heterodimeric amino acid transporters (HATs). CD98, linked to various light chains by disulfide bond, is responsible for cell surface expression and basolateral localization of this transporter complex in polarized epithelial cells and also interacts with beta1 integrins and increases their affinity for ligand. Besides its roles in amino acid transport, CD98 is thus involved in cell fusion and activation. It is implicated in regulation of cellular differentiation, growth and apoptosis.
Function Required for the function of light chain amino-acid transporters. Involved in sodium-independent, high-affinity transport of large neutral amino acids such as phenylalanine, tyrosine, leucine, arginine and tryptophan. Involved in guiding and targeting of LAT1 and LAT2 to the plasma membrane. When associated with SLC7A6 or SLC7A7 acts as an arginine/glutamine exchanger, following an antiport mechanism for amino acid transport, influencing arginine release in exchange for extracellular amino acids. Plays a role in nitric oxide synthesis in human umbilical vein endothelial cells (HUVECs) via transport of L-arginine. Required for normal and neoplastic cell growth. When associated with SLC7A5/LAT1, is also involved in the transport of L-DOPA across the blood-brain barrier, and that of thyroid hormones triiodothyronine (T3) and thyroxine (T4) across the cell membrane in tissues such as placenta. Involved in the uptake of methylmercury (MeHg) when administered as the L-cysteine or D,L-homocysteine complexes, and hence plays a role in metal ion homeostasis and toxicity. When associated with SLC7A5 or SLC7A8, involved in the cellular activity of small molecular weight nitrosothiols, via the stereoselective transport of L-nitrosocysteine (L-CNSO) across the transmembrane. Together with ICAM1, regulates the transport activity LAT2 in polarized intestinal cells, by generating and delivering intracellular signals. When associated with SLC7A5, plays an important role in transporting L-leucine from the circulating blood to the retina across the inner blood-retinal barrier. [UniProt]
Research Area Developmental Biology antibody; Immune System antibody; Metabolism antibody; Signaling Transduction antibody
Calculated MW 68 kDa
PTM Phosphorylation on Ser-406; Ser-408 or Ser-410 and on Ser-527 or Ser-531 by ecto-protein kinases favors heterotypic cell-cell interactions.

Images (2) Click the Picture to Zoom In

  • ARG53917 anti-CD98 antibody [MEM-108] (PE) FACS image

    Flow Cytometry: Human peripheral blood cells stained with ARG53917 anti-CD98 antibody [MEM-108] (PE).

  • ARG53917 anti-CD98 antibody [MEM-108] (PE) FACS image

    Flow Cytometry: Human peripheral blood monocytes stained with ARG53917 anti-CD98 antibody [MEM-108] (PE), followed by incubation with FITC labelled Goat anti-Mouse secondary antibody.