anti-CD99 antibody [12E7]

anti-CD99 antibody [12E7] for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation and Human

Cancer antibody; Immune System antibody; Signaling Transduction antibody


Product Description

Mouse Monoclonal antibody [12E7] recognizes CD99

Tested Reactivity Hu
Tested Application FACS, ICC/IF, IHC-P, IP
Host Mouse
Clonality Monoclonal
Clone 12E7
Isotype IgG1
Target Name CD99
Immunogen Acute lymphocytic leukemia T-cells.
Conjugation Un-conjugated
Alternate Names 12E7; CD99 antigen; MIC2X; MIC2Y; CD antigen CD99; MSK5X; Protein MIC2; MIC2; T-cell surface glycoprotein E2; HBA71; E2 antigen

Application Instructions

Application Suggestion
Tested Application Dilution
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.


Form Liquid
Purification Purified Antibody
Buffer 1X PBS and 0.1% Sodium azide
Preservative 0.1% Sodium azide
Concentration 0.2 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.


Database Links

GeneID: 4267 Human CD99

Swiss-port # P14209 Human CD99 antigen

Gene Symbol CD99
Gene Full Name CD99 molecule
Background CD99 is a cell surface glycoprotein involved in leukocyte migration, T-cell adhesion, ganglioside GM1 and transmembrane protein transport, and T-cell death by a caspase-independent pathway. In addition, the encoded protein may have the ability to rearrange the actin cytoskeleton and may also act as an oncosuppressor in osteosarcoma. This gene is found in the pseudoautosomal region of chromosomes X and Y and escapes X-chromosome inactivation. There is a related pseudogene located immediately adjacent to this locus. [provided by RefSeq, Mar 2016]
Function CD99 involved in T-cell adhesion processes and in spontaneous rosette formation with erythrocytes. Plays a role in a late step of leukocyte extravasation helping leukocytes to overcome the endothelial basement membrane. Acts at the same site as, but independently of, PECAM1. Involved in T-cell adhesion processes. [UniProt]
Research Area Cancer antibody; Immune System antibody; Signaling Transduction antibody
Calculated MW 19 kDa
PTM Extensively O-glycosylated.

Clone References

Pharmacokinetic modeling optimizes inhibition of the 'undruggable' EWS-FLI1 transcription factor in Ewing Sarcoma.

Hong SH et al.
Oncotarget.,  (2014)