anti-CD99 antibody [3B2/TA8] (PE)

anti-CD99 antibody [3B2/TA8] (PE) for Flow cytometry and Human

Cancer antibody; Immune System antibody; Signaling Transduction antibody


Product Description

PE-conjugated Mouse Monoclonal antibody [3B2/TA8] recognizes CD99

Tested Reactivity Hu
Tested Application FACS
Specificity The clone 3B2/TA8 recognizes CD99, an approximately 32 kDa sialoglycoprotein expressed on many cell types, with particularly strong expression on Ewing´s sarcoma and peripheral primitive neuroectodermal tumors. Within the hematopoietic system, CD99 is expressed on virtually all cell types except granulocytes.
HLDA VI.; WS Code T 6T-097, BP 534
Host Mouse
Clonality Monoclonal
Clone 3B2/TA8
Isotype IgG2a
Target Name CD99
Antigen Species Human
Immunogen Human thymocytes
Conjugation PE
Alternate Names 12E7; CD99 antigen; MIC2X; MIC2Y; CD antigen CD99; MSK5X; Protein MIC2; MIC2; T-cell surface glycoprotein E2; HBA71; E2 antigen

Application Instructions

Application Suggestion
Tested Application Dilution
FACS10 µl / 10^6 cells
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.


Form Liquid
Purification Note The purified antibody is conjugated with R-Phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary.
Buffer PBS, 15 mM Sodium azide and 0.2% (w/v) high-grade protease free BSA
Preservative 15 mM Sodium azide
Stabilizer 0.2% (w/v) high-grade protease free BSA
Storage Instruction Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.


Database Links

GeneID: 4267 Human CD99

Swiss-port # P14209 Human CD99 antigen

Gene Symbol CD99
Gene Full Name CD99 molecule
Background CD99 is a cell surface glycoprotein involved in leukocyte migration, T-cell adhesion, ganglioside GM1 and transmembrane protein transport, and T-cell death by a caspase-independent pathway. In addition, the encoded protein may have the ability to rearrange the actin cytoskeleton and may also act as an oncosuppressor in osteosarcoma. This gene is found in the pseudoautosomal region of chromosomes X and Y and escapes X-chromosome inactivation. There is a related pseudogene located immediately adjacent to this locus. [provided by RefSeq, Mar 2016]
Function CD99 involved in T-cell adhesion processes and in spontaneous rosette formation with erythrocytes. Plays a role in a late step of leukocyte extravasation helping leukocytes to overcome the endothelial basement membrane. Acts at the same site as, but independently of, PECAM1. Involved in T-cell adhesion processes. [UniProt]
Research Area Cancer antibody; Immune System antibody; Signaling Transduction antibody
Calculated MW 19 kDa
PTM Extensively O-glycosylated.

Images (2) Click the Picture to Zoom In

  • ARG54200 anti-CD99 antibody [3B2/TA8] (PE) FACS image

    Flow Cytometry: Human peripheral whole blood stained with ARG54200 anti-CD99 antibody [3B2/TA8] (PE) (10 µl reagent / 100 µl of peripheral whole blood).

  • ARG54200 anti-CD99 antibody [3B2/TA8] (PE) FACS image

    Flow Cytometry: Separation of human CD99 positive lymphocytes (red-filled) from neutrophil granulocytes (black-dashed). Human peripheral whole blood stained with ARG54200 anti-CD99 antibody [3B2/TA8] (PE) (10 µl reagent / 100 µl of peripheral whole blood).

Clone References

Lipid rafts and pseudotyping.

Pickl WF et al.
J Virol.,  (2001)




CD99 engagement on human peripheral blood T cells results in TCR/CD3-dependent cellular activation and allows for Th1-restricted cytokine production.

Waclavicek M et al.
J Immunol.,  (1998)