ARG66203

anti-CD99 antibody [SQab1725]

anti-CD99 antibody [SQab1725] for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human

Overview

Product Description

Recombinant Rabbit Monoclonal antibody [SQab1725] recognizes CD99

Tested Reactivity Hu
Tested Application IHC-P, WB
Host Rabbit
Clonality Monoclonal
Clone SQab1725
Target Name CD99
Antigen Species Human
Immunogen Synthetic peptide around the C‐terminus of CD99.
Conjugation Un-conjugated
Full Name CD99 molecule
Alternate Names 12E7; CD99 antigen; MIC2X; MIC2Y; CD antigen CD99; MSK5X; Protein MIC2; MIC2; T-cell surface glycoprotein E2; HBA71; E2 antigen

Application Instructions

Application Suggestion
Tested Application Dilution
IHC-P1:500 ‐ 1:1000
WB1:500
Application Note IHC-P: Antigen Retrieval: Heat mediation was performed using Citrate buffer (pH 6.0)
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Calculated MW 19 kDa

Properties

Form Liquid
Purification Affinity purification with immunogen.
Buffer PBS, 0.01% Sodium azide, 40% Glycerol and 0.05% BSA.
Preservative 0.01% Sodium azide
Stabilizer 40% Glycerol and 0.05% BSA
Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database links

GeneID: 4267 Human CD99

Swiss-port # P14209 Human CD99 antigen

Gene Symbol CD99
Gene Full Name CD99 molecule
Background CD99 is a cell surface glycoprotein involved in leukocyte migration, T-cell adhesion, ganglioside GM1 and transmembrane protein transport, and T-cell death by a caspase-independent pathway. In addition, the encoded protein may have the ability to rearrange the actin cytoskeleton and may also act as an oncosuppressor in osteosarcoma. This gene is found in the pseudoautosomal region of chromosomes X and Y and escapes X-chromosome inactivation. There is a related pseudogene located immediately adjacent to this locus. [provided by RefSeq, Mar 2016]
Function CD99 involved in T-cell adhesion processes and in spontaneous rosette formation with erythrocytes. Plays a role in a late step of leukocyte extravasation helping leukocytes to overcome the endothelial basement membrane. Acts at the same site as, but independently of, PECAM1. Involved in T-cell adhesion processes. [UniProt]
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Images (3) Click the Picture to Zoom In

  • ARG66203 anti-CD99 antibody [SQab1725] WB image

    Western blot: 30 µg of HeLa and Molt4 cell lysates stained with ARG66203 anti-CD99 antibody [SQab1725] at 1:500 dilution.

  • ARG66203 anti-CD99 antibody [SQab1725] IHC-P image

    Immunohistochemistry: Formalin‐fixed and paraffin‐embedded Human pancreas tissue stained with ARG66203 anti-CD99 antibody [SQab1725] at 1:500 dilution.

    Antigen retrieval: Heat mediated was performed using Citrate buffer pH 6.0

  • ARG66203 anti-CD99 antibody [SQab1725] IHC-P image

    Immunohistochemistry: Formalin‐fixed and paraffin‐embedded Human tonsil tissue stained with ARG66203 anti-CD99 antibody [SQab1725] at 1:500 dilution.

    Antigen retrieval: Heat mediated was performed using Citrate buffer pH 6.0