anti-CD99R antibody [MEM-131] (PE)

anti-CD99R antibody [MEM-131] (PE) for Flow cytometry and Human

Cancer antibody; Immune System antibody; Signaling Transduction antibody


Product Description

PE-conjugated Mouse Monoclonal antibody [MEM-131] recognizes CD99R

Tested Reactivity Hu
Tested Application FACS
Specificity The clone MEM-131 reacts with CD99R, an epitope restricted to a subset of CD99 molecule expressed on myeloid cells, NK cells and T lymphocytes.
HLDA V; WS Code AS S020
HLDA V; WS Code T T-E2.02
HLDA V; WS Code T T-017
Host Mouse
Clonality Monoclonal
Clone MEM-131
Isotype IgM
Target Name CD99R
Antigen Species Human
Immunogen HPB-ALL human peripheral blood leukemia T-cell line
Conjugation PE
Alternate Names 12E7; CD99 antigen; MIC2X; MIC2Y; CD antigen CD99; MSK5X; Protein MIC2; MIC2; T-cell surface glycoprotein E2; HBA71; E2 antigen

Application Instructions

Application Suggestion
Tested Application Dilution
FACS20 µl / 10^6 cells
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.


Form Liquid
Purification Note The purified antibody is conjugated with R-Phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary.
Buffer TBS, 15 mM Sodium azide and 0.2% (w/v) high-grade protease free BSA
Preservative 15 mM Sodium azide
Stabilizer 0.2% (w/v) high-grade protease free BSA
Storage Instruction Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.


Database Links

GeneID: 4267 Human CD99

Swiss-port # P14209 Human CD99 antigen

Gene Symbol CD99
Gene Full Name CD99 molecule
Background The protein encoded by this gene is a cell surface glycoprotein involved in leukocyte migration, T-cell adhesion, ganglioside GM1 and transmembrane protein transport, and T-cell death by a caspase-independent pathway. In addition, the encoded protein may have the ability to rearrange the actin cytoskeleton and may also act as an oncosuppressor in osteosarcoma. Cyclophilin A binds to CD99 and may act as a signaling regulator of CD99. This gene is found in the pseudoautosomal region of chromosomes X and Y and escapes X-chromosome inactivation. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2013]
Function Involved in T-cell adhesion processes and in spontaneous rosette formation with erythrocytes. Plays a role in a late step of leukocyte extravasation helping leukocytes to overcome the endothelial basement membrane. Acts at the same site as, but independently of, PECAM1. Involved in T-cell adhesion processes (By similarity). [UniProt]
Research Area Cancer antibody; Immune System antibody; Signaling Transduction antibody
Calculated MW 19 kDa
PTM Extensively O-glycosylated.

Images (1) Click the Picture to Zoom In

  • ARG53918 anti-CD99R antibody [MEM-131] (PE) FACS image

    Flow Cytometry: Human peripheral blood stained with ARG53918 anti-CD99R antibody [MEM-131] (PE).