ARG51620

anti-CDC25C phospho (Ser216) antibody

anti-CDC25C phospho (Ser216) antibody for Western blot,IHC-Formalin-fixed paraffin-embedded sections,ICC/IF and Human

Cell Biology and Cellular Response antibody; Gene Regulation antibody

Overview

Product Description Rabbit Polyclonal antibody recognizes CDC25C phospho (Ser216)
Tested Reactivity Hu
Tested Application ICC/IF, IHC-P, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name CDC25C
Antigen Species Human
Immunogen Peptide sequence around phosphorylation site of serine 216 (S-P-S(p)-M-P) derived from Human cdc25C.
Conjugation Un-conjugated
Alternate Names PPP1R60; M-phase inducer phosphatase 3; CDC25; EC 3.1.3.48; Dual specificity phosphatase Cdc25C

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IF1:100 - 1:200
IHC-P1:50 - 1:100
WB1:500 - 1:1000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Antibodies were produced by immunizing rabbits with KLH-conjugated synthetic phosphopeptide. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. In addition, non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Buffer PBS (without Mg2+ and Ca2+, pH 7.4), 150mM NaCl, 0.02% Sodium azide and 50% Glycerol.
Preservative 0.02% Sodium azide
Stabilizer 50% Glycerol
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 995 Human CDC25C

Swiss-port # P30307 Human M-phase inducer phosphatase 3

Gene Symbol CDC25C
Gene Full Name cell division cycle 25C
Background cdc25C is highly conserved during evolution and it plays a key role in the regulation of cell division. The encoded protein is a tyrosine phosphatase and belongs to the Cdc25 phosphatase family. It directs dephosphorylation of cyclin B-bound CDC2 and triggers entry into mitosis. It is also thought to suppress p53-induced growth arrest. Multiple alternatively spliced transcript variants of this gene have been described, however, the full-length nature of many of them is not known.
Function Functions as a dosage-dependent inducer in mitotic control. Tyrosine protein phosphatase required for progression of the cell cycle. When phosphorylated, highly effective in activating G2 cells into prophase. Directly dephosphorylates CDK1 and activates its kinase activity. [UniProt]
Research Area Cell Biology and Cellular Response antibody; Gene Regulation antibody
Calculated MW 53 kDa
PTM Phosphorylated by CHEK1 and MAPK14 at Ser-216. This phosphorylation creates a binding site for 14-3-3 protein and inhibits the phosphatase. Phosphorylated by PLK4. Phosphorylated by PLK1, leading to activate the phosphatase activity. Phosphorylation by PLK3 at Ser-191 promotes nuclear translocation. Ser-198 is a minor phosphorylation site. Was initially reported to be phosphorylated by PLK3 at Ser-216 (PubMed:10557092). However, such phosphorylation by PLK3 was not confirmed by other groups. Phosphorylation at Thr-48, Thr-67, Ser-122, Thr-130, Ser-168 and Ser-214 occurs at G2 and G2-M transition and is probably catalyzed by CDK1. Ser-168 phosphorylation levels are lower than those at the other 5 CDK1 sites. Phosphorylation by CDK1 leads to increased activity.

Images (3) Click the Picture to Zoom In

  • ARG51620 anti-CDC25C phospho (Ser216) antibody WB image

    Western blot: Extracts from 293 cells untreated or treated with serum stained with ARG51620 anti-CDC25C phospho (Ser216) antibody.

  • ARG51620 anti-CDC25C phospho (Ser216) antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Human breast carcinoma tissue stained with ARG51620 anti-CDC25C phospho (Ser216) antibody (left) or the same antibody preincubated with blocking peptide (right).

  • ARG51620 anti-CDC25C phospho (Ser216) antibody ICC/IF image

    Immunofluorescence: methanol-fixed HeLa cells stained with ARG51620 anti-CDC25C phospho (Ser216) antibody.