anti-CDK5 antibody [1H3]
anti-CDK5 antibody [1H3] for Western blot,Immunohistochemistry and Human,Mouse,Rat
Cancer antibody; Cell Biology and Cellular Response antibody; Metabolism antibody; Neuroscience antibody
|Product Description||Mouse Monoclonal antibody [1H3] recognizes CDK5|
|Tested Reactivity||Hu, Ms, Rat|
|Tested Application||IHC, WB|
|Immunogen||Purified rat Cdk5|
|Alternate Names||Cell division protein kinase 5; TPKII catalytic subunit; LIS7; PSSALRE; Serine/threonine-protein kinase PSSALRE; Cyclin-dependent-like kinase 5; EC 184.108.40.206; Tau protein kinase II catalytic subunit|
|Application Note||The antibody is specific for the ~ 28kDa Cdk5 protein in Western blots of Rat striatal lysate. The antibody shows no cross reactivity with the Cdk5 cofactor p35 or its degradation product p25. This monoclonal also works well for immunocytochemistry using primary cultured Rat neurons and for immunohistochemistry on mouse brain tissue.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
|Purification||Protein G purified|
|Buffer||10 mM HEPES (pH 7.5), 150 mM NaCl, 0.1 mg/ml BSA and 50% Glycerol|
|Stabilizer||0.1 mg/ml BSA, 50% Glycerol|
|Storage Instruction||For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.|
|Note||For laboratory research only, not for drug, diagnostic or other use.|
|Gene Full Name||cyclin-dependent kinase 5|
|Background||The neuronal protein kinase, Cdk5 has been implicated in a vast array of normal neuronal functions including regulation of neurotransmitter synthesis (Kansy J et al., 2004), the presynaptic vesicle cycle (Nguyen, C. & Bibb, JA 2003), neurotransmitter receptor trafficking and dopamine neurotransmission (Bibb, JA et al. 1999). At the same time Cdk5 has been implicated in a plethora of neurological and neuropsychiatric disorders including Alzheimer’s, Parkinson’s, Huntington’s, epilepsy, schizophrenia, and drug addiction. Detection of Cdk5 in normal samples as well as tissue undergoing neurodegeneration may advance studies in these areas. Moreover, this antibody may allow more accurate postmortem evaluations of Cdk5 protein expression, and thus serve as a valuable new reagent for neuropathology.|
|Research Area||Cancer antibody; Cell Biology and Cellular Response antibody; Metabolism antibody; Neuroscience antibody|
|Calculated MW||33 kDa|
|PTM||Phosphorylation on Tyr-15 by ABL1 and FYN, and on Ser-159 by casein kinase 1 promotes kinase activity. By contrast, phosphorylation at Thr-14 inhibits activity.
Phosphorylation at Ser-159 is essential for maximal catalytic activity.
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