ARG52240

anti-CDK5 antibody [1H3]

anti-CDK5 antibody [1H3] for Western blot,Immunohistochemistry and Human,Mouse,Rat

Cancer antibody; Cell Biology and Cellular Response antibody; Metabolism antibody; Neuroscience antibody

Overview

Product Description Mouse Monoclonal antibody [1H3] recognizes CDK5
Tested Reactivity Hu, Ms, Rat
Tested Application IHC, WB
Host Mouse
Clonality Monoclonal
Clone 1H3
Isotype IgG1
Target Name CDK5
Antigen Species Rat
Immunogen Purified rat Cdk5
Conjugation Un-conjugated
Alternate Names Cell division protein kinase 5; TPKII catalytic subunit; LIS7; PSSALRE; Serine/threonine-protein kinase PSSALRE; Cyclin-dependent-like kinase 5; EC 2.7.11.1; Tau protein kinase II catalytic subunit

Application Instructions

Application Suggestion
Tested Application Dilution
IHC1:500
WB1:1,000
Application Note The antibody is specific for the ~ 28kDa Cdk5 protein in Western blots of Rat striatal lysate. The antibody shows no cross reactivity with the Cdk5 cofactor p35 or its degradation product p25. This monoclonal also works well for immunocytochemistry using primary cultured Rat neurons and for immunohistochemistry on mouse brain tissue.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Protein G purified
Buffer 10 mM HEPES (pH 7.5), 150 mM NaCl, 0.1 mg/ml BSA and 50% Glycerol
Stabilizer 0.1 mg/ml BSA, 50% Glycerol
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 1020 Human CDK5

GeneID: 12568 Mouse CDK5

GeneID: 140908 ID: CDK5

Gene Symbol CDK5
Gene Full Name cyclin-dependent kinase 5
Background The neuronal protein kinase, Cdk5 has been implicated in a vast array of normal neuronal functions including regulation of neurotransmitter synthesis (Kansy J et al., 2004), the presynaptic vesicle cycle (Nguyen, C. & Bibb, JA 2003), neurotransmitter receptor trafficking and dopamine neurotransmission (Bibb, JA et al. 1999). At the same time Cdk5 has been implicated in a plethora of neurological and neuropsychiatric disorders including Alzheimer’s, Parkinson’s, Huntington’s, epilepsy, schizophrenia, and drug addiction. Detection of Cdk5 in normal samples as well as tissue undergoing neurodegeneration may advance studies in these areas. Moreover, this antibody may allow more accurate postmortem evaluations of Cdk5 protein expression, and thus serve as a valuable new reagent for neuropathology.
Research Area Cancer antibody; Cell Biology and Cellular Response antibody; Metabolism antibody; Neuroscience antibody
Calculated MW 33 kDa
PTM Phosphorylation on Tyr-15 by ABL1 and FYN, and on Ser-159 by casein kinase 1 promotes kinase activity. By contrast, phosphorylation at Thr-14 inhibits activity.
Phosphorylation at Ser-159 is essential for maximal catalytic activity.

Images (1) Click the Picture to Zoom In

  • ARG52240 anti-CDK5 antibody [1H3] WB image

    Western Blot: rat caudate lysate showing specific immunolabeling of the ~28k Cdk5 protein stained with CDK5 antibody [1H3] (ARG52240).