ARG51830

anti-CDK6 phospho (Tyr24) antibody

anti-CDK6 phospho (Tyr24) antibody for Western blot,IHC-Formalin-fixed paraffin-embedded sections,ICC/IF and Human,Mouse

Cancer antibody; Cell Biology and Cellular Response antibody; Gene Regulation antibody

Overview

Product Description Rabbit Polyclonal antibody recognizes CDK6 phospho (Tyr24)
Tested Reactivity Hu, Ms
Tested Application ICC/IF, IHC-P, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name CDK6
Antigen Species Human
Immunogen Peptide sequence around phosphorylation site of tyrosine 24 (G-A-Y(p)-G-K) derived from Human CDK6.
Conjugation Un-conjugated
Alternate Names Cell division protein kinase 6; PLSTIRE; EC 2.7.11.22; Serine/threonine-protein kinase PLSTIRE; MCPH12; Cyclin-dependent kinase 6

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IF1:100 - 1:200
IHC-P1:50 - 1:100
WB1:500 - 1:1000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Antibodies were produced by immunizing rabbits with KLH-conjugated synthetic phosphopeptide. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. In addition, non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Buffer PBS (without Mg2+ and Ca2+, pH 7.4), 150mM NaCl, 0.02% Sodium azide and 50% Glycerol.
Preservative 0.02% Sodium azide
Stabilizer 50% Glycerol
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 1021 Human CDK6

GeneID: 12571 Mouse CDK6

Swiss-port # Q00534 Human Cyclin-dependent kinase 6

Swiss-port # Q64261 Mouse Cyclin-dependent kinase 6

Gene Symbol CDK6
Gene Full Name cyclin-dependent kinase 6
Background Probably involved in the control of the cell cycle. Interacts with D-type G1 cyclins.
Function Serine/threonine-protein kinase involved in the control of the cell cycle and differentiation; promotes G1/S transition. Phosphorylates pRB/RB1 and NPM1. Interacts with D-type G1 cyclins during interphase at G1 to form a pRB/RB1 kinase and controls the entrance into the cell cycle. Involved in initiation and maintenance of cell cycle exit during cell differentiation; prevents cell proliferation and regulates negatively cell differentiation, but is required for the proliferation of specific cell types (e.g. erythroid and hematopoietic cells). Essential for cell proliferation within the dentate gyrus of the hippocampus and the subventricular zone of the lateral ventricles. Required during thymocyte development. Promotes the production of newborn neurons, probably by modulating G1 length. Promotes, at least in astrocytes, changes in patterns of gene expression, changes in the actin cytoskeleton including loss of stress fibers, and enhanced motility during cell differentiation. Prevents myeloid differentiation by interfering with RUNX1 and reducing its transcription transactivation activity, but promotes proliferation of normal myeloid progenitors. Delays senescence. Promotes the proliferation of beta-cells in pancreatic islets of Langerhans. May play a role in the centrosome organization during the cell cycle phases (PubMed:23918663). [UniProt]
Research Area Cancer antibody; Cell Biology and Cellular Response antibody; Gene Regulation antibody
Calculated MW 37 kDa
PTM Thr-177 phosphorylation and Tyr-24 dephosphorylation promotes kinase activity.

Images (3) Click the Picture to Zoom In

  • ARG51830 anti-CDK6 phospho (Tyr24) antibody WB image

    Western blot: Extracts from 293 cells untreated(lane 1) or treated with starvation(lane 2) stained with ARG51830 anti-CDK6 phospho (Tyr24) antibody.

  • ARG51830 anti-CDK6 phospho (Tyr24) antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Human colon carcinoma tissue stained with ARG51830 anti-CDK6 phospho (Tyr24) antibody (left) or the same antibody preincubated with blocking peptide (right).

  • ARG51830 anti-CDK6 phospho (Tyr24) antibody ICC/IF image

    Immunofluorescence: methanol-fixed HeLa cells stained with ARG51830 anti-CDK6 phospho (Tyr24) antibody.