ARG54113

anti-CDX2 antibody

anti-CDX2 antibody for ICC/IF,Immunoprecipitation,Western blot and Human,Monkey,Mouse

Controls and Markers antibody; Developmental Biology antibody; Gene Regulation antibody
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Overview

Product Description

Mouse Monoclonal antibody recognizes CDX2

Tested Reactivity Hu, Ms, Mk
Tested Application ICC/IF, IP, WB
Host Mouse
Clonality Monoclonal
Isotype IgG2b
Target Name CDX2
Antigen Species Human
Immunogen Purified recombinant human CDX2 protein fragments expressed in E.coli
Conjugation Un-conjugated
Alternate Names CDX2/AS; CDX3; Homeobox protein CDX-2; CDX-3; Caudal-type homeobox protein 2

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IF1:100
IPAssay-dependent
WB1:500 - 1:2000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Affinity purified
Buffer PBS (pH 7.4), 0.2% Sodium azide and 50% Glycerol
Preservative 0.2% Sodium azide
Stabilizer 50% Glycerol
Concentration 1.5 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 1045 Human CDX2

GeneID: 12591 Mouse CDX2

Swiss-port # P43241 Mouse Homeobox protein CDX-2

Swiss-port # Q99626 Human Homeobox protein CDX-2

Gene Symbol CDX2
Gene Full Name caudal type homeobox 2
Background Involved in the transcriptional regulation of multiple genes expressed in the intestinal epithelium.Important in broad range of functions from early differentiation to maintenance of the intestinal epithelial lining of both the small and large intestine.
Function Involved in the transcriptional regulation of multiple genes expressed in the intestinal epithelium. Important in broad range of functions from early differentiation to maintenance of the intestinal epithelial lining of both the small and large intestine. [UniProt]
Cellular Localization Nucleus
Research Area Controls and Markers antibody; Developmental Biology antibody; Gene Regulation antibody
Calculated MW 34 kDa
PTM Phosphorylation of Ser-60 mediates the transactivation capacity.

Images (3) Click the Picture to Zoom In

  • ARG54113 anti-CDX2 antibody ICC/IF image

    Immunofluorescence: 100% Methanol fixed (RT, 10 min) HeLa cells stained with ARG54113 anti-CDX2 antibody at 1:100 dilution. Left: primary antibody (green). Right: DAPI (blue).

    Secondary antibody: ARG55393 Goat anti-Mouse IgG (H+L) antibody (FITC)

  • ARG54113 anti-CDX2 antibody WB image

    Western blot: 30 µg of NIH3T3 cell lysate stained with ARG54113 anti-CDX2 antibody at 1:1000 dilution.

  • ARG54113 anti-CDX2 antibody IP image

    Immunoprecipitation: HeLa cell lysate were immunoprecipitated and stained with ARG54113 anti-CDX2 antibody.

Customer's Feedback

nuts_pic      Excellent

Review for anti-CDX2 antibody

Application:IF/ICC

Sample:HeLa

Fixation Buffer:100% Methanol

Fixation Time:10 min

Fixation Temperature:RT ºC

Permeabilization Buffer:0.1% Triton X-100

Primary Antibody Dilution Factor:1:100

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC

Conjugation of Secondary Antibody:FITC

nuts_pic      Excellent

Review for anti-CDX2 antibody

Application:WB

Sample:NIH3T3

Sample Loading Amount:30 µg

Primary Antibody Dilution Factor:1:1000

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC

nuts_pic      Average

Review for anti-CDX2 antibody

Application:WB

Sample:293T

Sample Loading Amount:30 µg

Primary Antibody Dilution Factor:1:1000

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC