ARG52839

anti-CDX2 antibody [SP54]

anti-CDX2 antibody [SP54] for IHC-Formalin-fixed paraffin-embedded sections,Flow cytometry and Human

Controls and Markers antibody; Developmental Biology antibody; Gene Regulation antibody

Overview

Product Description Rabbit Monoclonal antibody [SP54] recognizes CDX2
Tested Reactivity Hu
Tested Application FACS, IHC-P
Host Rabbit
Clonality Monoclonal
Clone SP54
Isotype IgG
Target Name CDX2
Antigen Species Human
Immunogen Synthetic peptide corresponding to N-terminus of human CDX2.
Conjugation Un-conjugated
Alternate Names CDX2/AS; CDX3; Homeobox protein CDX-2; CDX-3; Caudal-type homeobox protein 2

Application Instructions

Application Suggestion
Tested Application Dilution
FACSAssay-Dependent
IHC-P1:100
Application Note IHC-P: Antigen Retrieval: Boil tissue section in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.
Incubation Time: 30 min at RT.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control Colon Carcinoma

Properties

Form Liquid
Purification Purified by protein A/G
Buffer PBS (pH 7.6), 1% BSA and < 0.1% Sodium azide
Preservative < 0.1% Sodium azide
Stabilizer 1% BSA
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 1045 Human CDX2

Swiss-port # Q99626 Human Homeobox protein CDX-2

Background CDX2 is a caudal type homeobox gene that encodes an intenstine-specific transcription factor that is expressed early in intestinal development and may be involved in the regulation of proliferation and differentiation of intestinal epithelial cells. It is expressed in the nuclei of epithelial cells throughout the intestine, from duodenum to rectum. The CDX2 protein is expressed in primary and metastatic colorectal carcinomas and has also been demonstrated in the intestinal metaplasia of the stomach and intestinal-type gastric cancer, while it is not expressed in the normal gastric mucosa. Studies have shown that CDX2 is superior marker compared in CK20.
Cellular Localization Nucleus
Highlight Related products:
CDX2 antibodies; Anti-Rabbit IgG secondary antibodies;
Related news:
Wnt / beta-catenin signaling for gastric fundus specification
Research Area Controls and Markers antibody; Developmental Biology antibody; Gene Regulation antibody
Calculated MW 34 kDa
PTM Phosphorylation of Ser-60 mediates the transactivation capacity.

Images (1) Click the Picture to Zoom In

  • ARG52839 anti-CDX2 antibody [SP54] IHC-P image

    Immunohistochemistry: Human Colon Adenoarcinoma stained with CDX2 antibody [SP54] (ARG52839)

Clone References

5-Aza-dC treatment induces mesenchymal-to-epithelial transition in 1st trimester trophoblast cell line HTR8/SVneo.

ICC/IF / Human

Chen Y et al.
Biochem Biophys Res Commun.,  (2013)

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