ARG66244

anti-CDX2 antibody [SQab1733]

anti-CDX2 antibody [SQab1733] for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human

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Overview

Product Description Recombinant Rabbit Monoclonal antibody [SQab1733] recognizes CDX2
Tested Reactivity Hu
Tested Application IHC-P, WB
Host Rabbit
Clonality Monoclonal
Clone SQab1733
Isotype IgG
Target Name CDX2
Antigen Species Human
Immunogen Synthetic peptide around the N-terminus of Human CDX2.
Conjugation Un-conjugated
Alternate Names CDX2/AS; CDX3; Homeobox protein CDX-2; CDX-3; Caudal-type homeobox protein 2

Application Instructions

Application Suggestion
Tested Application Dilution
IHC-P1:100 - 1:200
WB1:500 - 1:2000
Application Note IHC-P: Antigen Retrieval: Boil tissue section in Tris/EDTA buffer (pH 9.0).
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control SW480; Human colon tissue
Observed Size ~ 40 kDa

Properties

Form Liquid
Purification Purification with Protein A.
Buffer PBS, 0.01% Sodium azide, 40% Glycerol and 0.05% BSA.
Preservative 0.01% Sodium azide
Stabilizer 40% Glycerol and 0.05% BSA
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 1045 Human CDX2

Swiss-port # Q99626 Human Homeobox protein CDX-2

Gene Symbol CDX2
Gene Full Name caudal type homeobox 2
Background This gene is a member of the caudal-related homeobox transcription factor gene family. The encoded protein is a major regulator of intestine-specific genes involved in cell growth an differentiation. This protein also plays a role in early embryonic development of the intestinal tract. Aberrant expression of this gene is associated with intestinal inflammation and tumorigenesis. [provided by RefSeq, Jan 2012]
Function Involved in the transcriptional regulation of multiple genes expressed in the intestinal epithelium. Important in broad range of functions from early differentiation to maintenance of the intestinal epithelial lining of both the small and large intestine. [UniProt]
Highlight Related products:
CDX2 antibodies; Anti-Rabbit IgG secondary antibodies;
Related news:
Cancer Pathology Markers (SQ clones)
Calculated MW 34 kDa
PTM Phosphorylation of Ser-60 mediates the transactivation capacity. [UniProt]

Images (2) Click the Picture to Zoom In

  • ARG66244 anti-CDX2 antibody [SQab1733] IHC-P image

    Immunohistochemistry: Formalin-fixed and paraffin-embedded Human colon tissue stained with ARG66244 anti-CDX2 antibody [SQab1733] at 1:200. Antigen Retrieval: Boil tissue section in Tris/EDTA buffer (pH 9.0).

  • ARG66244 anti-CDX2 antibody [SQab1733] WB image

    Western blot: SW480 cell lysate stained with ARG66244 anti-CDX2 antibody [SQab1733] at 1:1000 dilution.

Customer's Feedback

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Review for anti-CDX2 antibody [SQab1733]

Application:WB

Sample:SW480

Sample Loading Amount:20 μg

Primary Antibody Dilution Factor:1:1000

Primary Antibody Incubation Time:Overnight

Primary Antibody Incubation Temperature:4 ºC

Conjugation of Secondary Antibody:HRP