ARG45701
anti-CENPB antibody
anti-CENPB antibody for Flow cytometry,ICC/IF,Western blot and Human
Overview
| Product Description | Rabbit Polyclonal antibody recognizes CENPB |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | FACS, ICC/IF, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | CENPB |
| Antigen Species | Human |
| Immunogen | Synthetic peptide corresponding to C-terminal region of human CENPB. |
| Conjugation | Un-conjugated |
| Alternate Names | CENPB; Centromere Protein B; Major Centromere Autoantigen B; Centromere Protein B, 80kDa; CENP-B; Centromere Protein B (80kD); Centromere Autoantigen B |
Application Instructions
| Application Suggestion |
|
||||||||
|---|---|---|---|---|---|---|---|---|---|
| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
| Observed Size | 80 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purified |
| Buffer | 0.2% Na2HPO4, 0.9% NaCl and 4% Trehalose. |
| Stabilizer | 4% Trehalose |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | CENPB |
| Gene Full Name | Centromere Protein B |
| Background | This gene product is a highly conserved protein that facilitates centromere formation. It is a DNA-binding protein that is derived from transposases of the pogo DNA transposon family. It contains a helix-loop-helix DNA binding motif at the N-terminus, and a dimerization domain at the C-terminus. The DNA binding domain recognizes and binds a 17-bp sequence (CENP-B box) in the centromeric alpha satellite DNA. This protein is proposed to play an important role in the assembly of specific centromere structures in interphase nuclei and on mitotic chromosomes. It is also considered a major centromere autoantigen recognized by sera from patients with anti-centromere antibodies. [provided by RefSeq, Jul 2008] |
| Function | Interacts with centromeric heterochromatin in chromosomes and binds to a specific 17 bp subset of alphoid satellite DNA, called the CENP-B box. [UniProt] |
| Cellular Localization | Centromere; Chromosome; Nucleus. [UniProt] |
| Calculated MW | 65 kDa |
| PTM | ADP-ribosylation; Isopeptide bond; Methylation; Phosphoprotein; Ubl conjugation. [UniProt] |
Images (3) Click the Picture to Zoom In
-
ARG45701 anti-CENPB antibody ICC/IF image
Immunofluorescence: MCF-7 stained with ARG45701 anti-CENPB antibody at 5 μg/ml dilution.
-
ARG45701 anti-CENPB antibody WB image
Western blot: MCF-7 and PC-3 stained with ARG45701 anti-CENPB antibody at 0.5 μg/ml dilution.
-
ARG45701 anti-CENPB antibody FACS image
Flow Cytometry: A431 stained with ARG45701 anti-CENPB antibody at 1 µg/10^6 cells dilution.
