ARG20118

anti-CHOP / GADD153 antibody

anti-CHOP / GADD153 antibody for Immunohistochemistry,Immunoprecipitation,Western blot and Human,Mouse,Rat

Developmental Biology antibody; Gene Regulation antibody; Metabolism antibody
publication_link Publication1

Overview

Product Description

Rabbit Polyclonal antibody recognizes CHOP / GADD153

Tested Reactivity Hu, Ms, Rat
Tested Application IHC, IP, WB
Specificity The antibody recognizes 25 kDa GADD153 of human, mouse, and rat origins.
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name CHOP / GADD153
Antigen Species Human
Immunogen Synthetic peptide surrounding amino acid 124 of human GADD153
Conjugation Un-conjugated
Alternate Names DNA damage-inducible transcript 3 protein; CHOP10; Growth arrest and DNA damage-inducible protein GADD153; GADD153; CCAAT/enhancer-binding protein homologous protein; C/EBP-homologous protein; CHOP; CHOP-10; C/EBP zeta; CEBPZ; DDIT-3; C/EBP-homologous protein 10

Application Instructions

Application Suggestion
Tested Application Dilution
IHC10-20 µg/ml
IP10-20 µg/ml
WB0.5-4 µg/ml
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Calculated MW 19 kDa

Properties

Form Liquid
Purification Affinity Purified Antibody
Buffer PBS, 30% Glycerol, 0.5% BSA and 0.01% Thimerosal
Preservative 0.01% Thimerosal
Stabilizer 30% Glycerol, 0.5% BSA
Concentration 0.2 mg/ml
Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database links

GeneID: 13198 Mouse DDIT3

GeneID: 1649 Human DDIT3

Swiss-port # P35638 Human DNA damage-inducible transcript 3 protein

Swiss-port # P35639 Mouse DNA damage-inducible transcript 3 protein

Background GADD153 has been described as a growth arrest and DNA damage-inducible gene that encodes a C/EBP-related nuclear protein. Expression of GADD153 is induced by a variety of cellular stresses, including nutrient deprivation and metabolic perturbations. GADD153 functions to block cells in G1 to S phase in cell cycle progression and acts by dimerizing with other C/EBP proteins to direct GADD153 dimers away from “classical” C/EBP binding sites. Thus GADD153 acts as a negative modulator of C/EBP-like proteins in certain terminally differentiated cells.
Research Area Developmental Biology antibody; Gene Regulation antibody; Metabolism antibody
PTM Ubiquitinated, leading to its degradation by the proteasome.
Phosphorylation at serine residues by MAPK14 enhances its transcriptional activation activity while phosphorylation at serine residues by CK2 inhibits its transcriptional activation activity.

Specific References

Mangiferin alleviates endoplasmic reticulum stress in acute liver injury by regulating the miR-20a/miR-101a-Nrf2 axis.

WB / Mouse

Shaoxun Li et al.
J Biochem.,  (2020)

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