ARG52899

anti-COX2 antibody

anti-COX2 antibody for ELISA,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,IHC-Frozen sections,Immunoprecipitation and Hamster,Human,Mouse,Rat

Inflammation Study antibody

Overview

Product Description Rabbit Polyclonal antibody recognizes COX2
Tested Reactivity Hu, Ms, Rat, Hm
Tested Application ELISA, ICC/IF, IHC-Fr, IHC-P, IP
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name COX2
Antigen Species Rat
Immunogen Synthetic peptide derived from C-terminus of rat COX-2.
Conjugation Un-conjugated
Alternate Names PHS II; Prostaglandin H2 synthase 2; PHS-2; Cyclooxygenase-2; PGHS-2; COX2; PGG/HS; COX-2; GRIPGHS; hCox-2; PGH synthase 2; Prostaglandin G/H synthase 2; Prostaglandin-endoperoxide synthase 2; EC 1.14.99.1

Application Instructions

Application Suggestion
Tested Application Dilution
ELISAAssay-Dependent
ICC/IFAssay-Dependent
IHC-FrAssay-Dependent
IHC-P1:100
IPAssay-Dependent
Application Note IHC-P: Antigen Retrieval: Boil tissue section in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.
Incubation Time: 10 min at RT.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control Breast Carcinoma, Colon, Lung

Properties

Form Liquid
Purification Immunogen affinity purified
Buffer PBS (pH 7.6), 1% BSA and < 0.1% Sodium azide
Preservative < 0.1% Sodium azide
Stabilizer 1% BSA
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 19225 Mouse PTGS2

GeneID: 29527 Rat PTGS2

GeneID: 5743 Human PTGS2

Gene Symbol Ptgs2
Gene Full Name prostaglandin-endoperoxide synthase 2
Background COX2: Prostaglandin-endoperoxide synthase (PTGS), also known as cyclooxygenase, is the key enzyme in prostaglandin biosynthesis, and acts both as a dioxygenase and as a peroxidase. There are two isozymes of PTGS: a constitutive PTGS1 and an inducible PTGS2, which differ in their regulation of expression and tissue distribution. This gene encodes the inducible isozyme. It is regulated by specific stimulatory events, suggesting that it is responsible for the prostanoid biosynthesis involved in inflammation and mitogenesis. [provided by RefSeq, Feb 2009]
Function COX2 converts arachidonate to prostaglandin H2 (PGH2), a committed step in prostanoid synthesis (PubMed:26859324, PubMed:27226593). Constitutively expressed in some tissues in physiological conditions, such as the endothelium, kidney and brain, and in pathological conditions, such as in cancer. PTGS2 is responsible for production of inflammatory prostaglandins. Up-regulation of PTGS2 is also associated with increased cell adhesion, phenotypic changes, resistance to apoptosis and tumor angiogenesis. In cancer cells, PTGS2 is a key step in the production of prostaglandin E2 (PGE2), which plays important roles in modulating motility, proliferation and resistance to apoptosis. During neuroinflammation, plays a role in neuronal secretion of specialized preresolving mediators (SPMs), especially 15-R-lipoxin A4, that regulates phagocytic microglia. [UniProt]
Cellular Localization Cytoplasm, Membrane
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Research Area Inflammation Study antibody
Calculated MW 69 kDa
PTM S-nitrosylation by NOS2 (iNOS) activates enzyme activity. S-nitrosylation may take place on different Cys residues in addition to Cys-526.

Images (1) Click the Picture to Zoom In

  • ARG52899 anti-COX2 antibody IHC-P image

    Immunohistochemistry: Human Breast Carcinoma stained with ARG52899 anti-COX2 antibody.