ARG54003

anti-COX4 antibody

anti-COX4 antibody for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation,Western blot and Goat,Hamster,Human,Monkey,Mouse,Rat

Cancer antibody; Controls and Markers antibody; Metabolism antibody; Signaling Transduction antibody; Loading Control antibody for Cytoplasmic Fractions; Cytochrome-C fractionation Study antibody; Mitochondrial Marker antibody
nuts_pic
3

Overview

Product Description Mouse Monoclonal antibody recognizes COX4
Tested Reactivity Hu, Ms, Rat, Goat, Hm, Mk
Tested Application FACS, ICC/IF, IHC-P, IP, WB
Host Mouse
Clonality Monoclonal
Isotype IgG1
Target Name COX4
Antigen Species Human
Immunogen A synthetic peptide corresponding to carboxyl terminal residues of human COX IV
Conjugation Un-conjugated
Alternate Names Cytochrome c oxidase polypeptide IV; COX4; COX4-1; COXIV; Cytochrome c oxidase subunit IV isoform 1; COX IV-1; Cytochrome c oxidase subunit 4 isoform 1, mitochondrial

Application Instructions

Application Suggestion
Tested Application Dilution
FACS1:100
ICC/IF1:150
IHC-P1:50
IPAssay-dependent
WB1:1000
Application Note IHC-P: Antigen Retrieval: High-pressure and temperature Citrate buffer (pH 6.0).
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Affinity purified
Buffer 0.1M Tris-Glycine (pH 7.4), 150 mM NaCl, 0.2% Sodium azide, 0.1mg/ml BSA and 50% Glycerol
Preservative 0.2% Sodium azide
Stabilizer 0.1mg/ml BSA, 50% Glycerol
Concentration 1mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 12857 Mouse COX4I1

GeneID: 1327 Human COX4I1

GeneID: 29445 Rat COX4I1

Gene Symbol COX4I1
Gene Full Name cytochrome c oxidase subunit IV isoform 1
Background Cytochrome c oxidase (COX) is the terminal enzyme of the mitochondrial respiratory chain. It is a multi-subunit enzyme complex that couples the transfer of electrons from cytochrome c to molecular oxygen and contributes to a proton electrochemical gradient across the inner mitochondrial membrane. The complex consists of 13 mitochondrial- and nuclear-encoded subunits. The mitochondrially-encoded subunits perform the electron transfer and proton pumping activities. The functions of the nuclear-encoded subunits are unknown but they may play a role in the regulation and assembly of the complex. This gene encodes the nuclear-encoded subunit IV isoform 1 of the human mitochondrial respiratory chain enzyme. It is located at the 3' of the NOC4 (neighbor of COX4) gene in a head-to-head orientation, and shares a promoter with it. Pseudogenes related to this gene are located on chromosomes 13 and 14. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jan 2016]
Function COX4 protein is one of the nuclear-coded polypeptide chains of cytochrome c oxidase, the terminal oxidase in mitochondrial electron transport. [UniProt]
Cellular Localization Mitochondrion inner membrane. [UniProt]
Highlight Related Antibody Duos and Panels:
ARG30259 Loading Controls for Cytoplasmic / Nuclear Fractions Antibody Panel
ARG30271 Mitochondrial Marker Antibody Panel (Cytochrome C, COX4, HSP60)
ARG30276 Cytochrome-C fractionation Antibody Panel (Cytochrome-C, COX IV, beta Actin)
Related products:
COX IV antibodies; COX IV Duos / Panels; Anti-Mouse IgG secondary antibodies;
Related poster download:
The Structure & Functions of Mitochondria.pdf
Research Area Cancer antibody; Controls and Markers antibody; Metabolism antibody; Signaling Transduction antibody; Loading Control antibody for Cytoplasmic Fractions; Cytochrome-C fractionation Study antibody; Mitochondrial Marker antibody
Calculated MW 20 kDa

Images (6) Click the Picture to Zoom In

  • ARG54003 anti-COX4 antibody ICC/IF image

    Immunofluorescence: 100% Methanol fixed (RT, 10 min) HeLa cells stained with ARG54003 anti-COX4 antibody (green) at 1:150 dilution.

    Secondary antibody: ARG55393 Goat anti-Mouse IgG (H+L) antibody (FITC)

  • ARG54003 anti-COX4 antibody FACS image

    Flow Cytometry: K562 cells stained with ARG54003 anti-COX4 antibody at 1:100 dilution (right histogram) or isotype control (left histogram), followed by incubation with FITC labelled secondary antibody.

  • ARG54003 anti-COX4 antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Human colorectal carcinoma stained with ARG54003 anti-COX4 antibody at 1:50 dilution. Antigen Retrieval: High-pressure and temperature Citrate buffer (pH 6.0).

  • ARG54003 anti-COX4 antibody IP image

    Immunoprecipitation: HeLa cell lysates were immunoprecipitated and stained with ARG54003 anti-COX4 antibody.

  • ARG54003 anti-COX4 antibody WB image

    Western blot: 20 µg of HeLa, Mouse brain and Rat brain lysates stained with ARG54003 anti-COX4 antibody at 1:1000 dilution.

  • ARG54003 anti-COX4 antibody ICC/IF image

    Immunofluorescence: 100% Methanol fixed (RT, 10 min) HeLa cells stained with ARG54003 anti-COX4 antibody (green) at 1:150 dilution.

    Secondary antibody: ARG55393 Goat anti-Mouse IgG (H+L) antibody (FITC)

Customer's Feedback

nuts_pic      Excellent

Review for anti-COX4 antibody

Application:IF/ICC

Sample:HeLa

Fixation Buffer:100% Methanol

Fixation Time:10 min

Fixation Temperature:RT ºC

Permeabilization Buffer:0.1% Triton X-100

Primary Antibody Dilution Factor:1:150

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC

Conjugation of Secondary Antibody:FITC

nuts_pic      Excellent

Review for anti-COX4 antibody

Application:WB

Sample:HeLa, Mouse brain and Rat brain

Sample Loading Amount:20 µg

Primary Antibody Dilution Factor:1:1000

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC

nuts_pic      Excellent

Review for anti-COX4 antibody

Application:WB

Sample:A: Mouse brain, B: Rat brain

Sample Loading Amount:30 µg

Primary Antibody Dilution Factor:1:1000

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC