ARG45459
anti-CPS1 antibody
anti-CPS1 antibody for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat,Monkey
Overview
| Product Description | Rabbit Polyclonal antibody recognizes CPS1 |
|---|---|
| Tested Reactivity | Hu, Ms, Rat, Mk |
| Tested Application | FACS, ICC/IF, IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | CPS1 |
| Antigen Species | Human |
| Immunogen | Recombinant protein containing to human CPS1. |
| Conjugation | Un-conjugated |
| Alternate Names | CPS1; CPSASE1; Carbamoyl-phosphate synthase [ammonia], mitochondrial; CPSase I; PHN; Carbamoyl-phosphate synthetase I; EC 6.3.4.16 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||||
| Observed Size | 165 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purified |
| Buffer | 0.2% Na2HPO4, 0.9% NaCl and 4% Trehalose. |
| Stabilizer | 4% Trehalose |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | CPS1 |
| Gene Full Name | carbamoyl-phosphate synthase 1, mitochondrial |
| Background | The mitochondrial enzyme encoded by this gene catalyzes synthesis of carbamoyl phosphate from ammonia and bicarbonate. This reaction is the first committed step of the urea cycle, which is important in the removal of excess urea from cells. The encoded protein may also represent a core mitochondrial nucleoid protein. Three transcript variants encoding different isoforms have been found for this gene. The shortest isoform may not be localized to the mitochondrion. Mutations in this gene have been associated with carbamoyl phosphate synthetase deficiency, susceptibility to persistent pulmonary hypertension, and susceptibility to venoocclusive disease after bone marrow transplantation.[provided by RefSeq, May 2010] |
| Function | Involved in the urea cycle of ureotelic animals where the enzyme plays an important role in removing excess ammonia from the cell. [UniProt] |
| Cellular Localization | Finely granular cytoplasmic. [UniProt] |
| Calculated MW | 165 kDa |
| PTM | Undergoes proteolytic cleavage in the C-terminal region corresponding to the loss of approximately 12 AA residues from the C-terminus. Succinylated at Lys-287 and Lys-1291. Desuccinylated at Lys-1291 by SIRT5, leading to activation (By similarity). Glutarylated. Glutarylation levels increase during fasting. Deglutarylated by SIRT5 at Lys-55, Lys-219, Lys-412, Lys-889, Lys-892, Lys-915, Lys-1360 and Lys-1486, leading to activation. [UniProt] |
Images (9) Click the Picture to Zoom In
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ARG45459 anti-CPS1 antibody IHC-P image
Immunohistochemistry: Human liver cancer stained with ARG45459 anti-CPS1 antibody at 2 μg/ml dilution.
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ARG45459 anti-CPS1 antibody ICC/IF image
Immunofluorescence: Hela stained with ARG45459 anti-CPS1 antibody at 5 μg/ml dilution.
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ARG45459 anti-CPS1 antibody WB image
Western blot: HCCT stained with ARG45459 anti-CPS1 antibody at 0.5 μg/ml dilution.
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ARG45459 anti-CPS1 antibody FACS image
Flow Cytometry: Hela stained with ARG45459 anti-CPS1 antibody at 1 µg/10^6 cells dilution.
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ARG45459 anti-CPS1 antibody IHC-P image
Immunohistochemistry: Rat colon stained with ARG45459 anti-CPS1 antibody at 2 μg/ml dilution.
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ARG45459 anti-CPS1 antibody WB image
Western blot: Rat liver stained with ARG45459 anti-CPS1 antibody at 0.5 μg/ml dilution.
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ARG45459 anti-CPS1 antibody IHC-P image
Immunohistochemistry: Mouse colon stained with ARG45459 anti-CPS1 antibody at 2 μg/ml dilution.
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ARG45459 anti-CPS1 antibody WB image
Western blot: Mouse liver stained with ARG45459 anti-CPS1 antibody at 0.5 μg/ml dilution.
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ARG45459 anti-CPS1 antibody WB image
Western blot: Monkey liver stained with ARG45459 anti-CPS1 antibody at 0.5 μg/ml dilution.
