ARG52253

anti-CREB phospho (Ser133) antibody

anti-CREB phospho (Ser133) antibody for Western blot,ICC/IF and Rat

Gene Regulation antibody; Immune System antibody; Signaling Transduction antibody

Overview

Product Description Rabbit Polyclonal antibody recognizes CREB phospho (Ser133)
Tested Reactivity Rat
Predict Reactivity Hu, Ms, Rat, Bov, Chk, Dog, NHuPrm, Sheep, Xenopus laevis, Zfsh
Tested Application ICC/IF, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name CREB
Antigen Species Rat
Immunogen Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser133 conjugated to KLH
Conjugation Un-conjugated
Alternate Names Cyclic AMP-responsive element-binding protein 1; CREB; CREB-1; cAMP-responsive element-binding protein 1

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IFAssay-dependent
WB1:1,000
Application Note Specific for the ~45k CREB protein phosphorylated at Ser133. Immunolabeling is blocked by λ-phosphatase treatment.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Affinity Purified
Buffer 10 mM HEPES (pH 7.5), 150 mM NaCl, 0.1 mg/ml BSA and 50% Glycerol
Stabilizer 0.1 mg/ml BSA, 50% Glycerol
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 81646 Rat CREB1

Swiss-port # P15337 Rat Cyclic AMP-responsive element-binding protein 1

Gene Symbol CREB1
Gene Full Name cAMP responsive element binding protein 1
Background CREB is a transcription factor. It is a member of the leucine zipper family of DNA binding proteins. This protein binds as a homodimer to the cAMP-responsive element, an octameric palindrome. The protein is phosphorylated by several protein kinases, and induces transcription of genes in response to hormonal stimulation of the cAMP pathway. Alternate splicing of this gene results in several transcript variants encoding different isoforms. [provided by RefSeq, Mar 2016]
Function CREB is a phosphorylation-dependent transcription factor. It stimulates transcription upon binding to the DNA cAMP response element (CRE), a sequence present in many viral and cellular promoters. Transcription activation is enhanced by the TORC coactivators which act independently of Ser-133 phosphorylation. Involved in different cellular processes including the synchronization of circadian rhythmicity and the differentiation of adipose cells. [UniProt]
Research Area Gene Regulation antibody; Immune System antibody; Signaling Transduction antibody
Calculated MW 37 kDa
PTM Stimulated by phosphorylation. Phosphorylation of both Ser-133 and Ser-142 in the SCN regulates the activity of CREB and participates in circadian rhythm generation. Phosphorylation of Ser-133 allows CREBBP binding. In liver, phosphorylation is induced by fasting or glucagon in a circadian fashion (By similarity). CREBL2 positively regulates phosphorylation at Ser-133 thereby stimulating CREB1 transcriptional activity (By similarity). Phosphorylated upon calcium influx by CaMK4 and CaMK2 on Ser-133. CaMK4 is much more potent than CaMK2 in activating CREB. Phosphorylated by CaMK2 on Ser-142. Phosphorylation of Ser-142 blocks CREB-mediated transcription even when Ser-133 is phosphorylated. Phosphorylated by CaMK1 (By similarity). Phosphorylation of Ser-271 by HIPK2 in response to genotoxic stress promotes CREB1 activity, facilitating the recruitment of the coactivator CBP. Phosphorylated at Ser-133 by RPS6KA3, RPS6KA4 and RPS6KA5 in response to mitogenic or stress stimuli.
Sumoylated with SUMO1. Sumoylation on Lys-304, but not on Lys-285, is required for nuclear localization of this protein. Sumoylation is enhanced under hypoxia, promoting nuclear localization and stabilization.

Images (2) Click the Picture to Zoom In

  • ARG52253 anti-CREB phospho (Ser133) antibody WB image

    Western blot: a forskolin-stimulated Rat hippocampal slice showing phospho-specific immunolabeling of the ~45k CREB protein phosphorylated at Ser 133 stained with ARG52253 anti-CREB phospho (Ser133) antibody.

  • ARG52253 anti-CREB phospho (Ser133) antibody ICC/IF image

    Immunofluorescence: Cultured Rat hippocampal slices stained with ARG52253 anti-CREB phospho (Ser133) antibody showing nuclear labeling of CREB phosphorylated at Ser133 in brown.