ARG45473
anti-CSK antibody
anti-CSK antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
Overview
| Product Description | Rabbit Polyclonal antibody recognizes CSK |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | CSK |
| Antigen Species | Human |
| Immunogen | Recombinant protein containing to human CSK. |
| Conjugation | Un-conjugated |
| Alternate Names | Protein-tyrosine kinase CYL; C-Src kinase; Tyrosine-protein kinase CSK; EC 2.7.10.2 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| Observed Size | 51 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purified |
| Buffer | 0.9% NaCl, 0.2% Na2HPO4, 0.05% Sodium azide and 5% BSA. |
| Preservative | 0.05% Sodium azide |
| Stabilizer | 5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | CSK |
| Gene Full Name | c-src tyrosine kinase |
| Background | C-terminal Src kinase (Csk) is a non-receptor protein tyrosine kinase which resembles Src-family kinases, but unlike them lacks the conserved autophosphorylation site, the regulatory C-terminal tyrosine as well as myristylation and palmitylation. Csk negatively regulates Src-family kinases by phosphorylation of their C-terminal regulatory tyrosine. Disruption of the csk gene causes constitutive activation of Src-family kinases, and overexpression of Csk usually counteracts their signaling. The Csk-mediated regulation of those Src-family kinases that are localized in lipid rafts is enabled by a ubiquitously expressed transmembrane adaptor PAG (also known as Cbp, Csk-binding protein), which recruits Csk. |
| Function | Non-receptor tyrosine-protein kinase that plays an important role in the regulation of cell growth, differentiation, migration and immune response. Phosphorylates tyrosine residues located in the C-terminal tails of Src-family kinases (SFKs) including LCK, SRC, HCK, FYN, LYN or YES1. Upon tail phosphorylation, Src-family members engage in intramolecular interactions between the phosphotyrosine tail and the SH2 domain that result in an inactive conformation. To inhibit SFKs, CSK is recruited to the plasma membrane via binding to transmembrane proteins or adapter proteins located near the plasma membrane. Suppresses signaling by various surface receptors, including T-cell receptor (TCR) and B-cell receptor (BCR) by phosphorylating and maintaining inactive several positive effectors such as FYN or LCK. [UniProt] |
| Cellular Localization | Cell membrane; Cytoplasm; Membrane. [UniProt] |
| Calculated MW | 51 kDa |
| PTM | Phosphorylated at Ser-364 by PKA, leading to increased activity. Autophosphorylated. [UniProt] |
Images (4) Click the Picture to Zoom In
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ARG45473 anti-CSK antibody IHC-P image
Immunohistochemistry: Human tonsil stained with ARG45473 anti-CSK antibody at 1 μg/ml dilution.
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ARG45473 anti-CSK antibody WB image
Western blot: Hela stained with ARG45473 anti-CSK antibody at 0.5 μg/ml dilution.
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ARG45473 anti-CSK antibody WB image
Western blot: Rat testis stained with ARG45473 anti-CSK antibody at 0.5 μg/ml dilution.
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ARG45473 anti-CSK antibody WB image
Western blot: Mouse liver stained with ARG45473 anti-CSK antibody at 0.5 μg/ml dilution.
