ARG10617

anti-CST3 / Cystatin C antibody [Cyst13]

anti-CST3 / Cystatin C antibody [Cyst13] for ELISA,FLISA,Western blot and Human

Overview

Product Description Mouse Monoclonal antibody [Cyst13] recognizes CST3 / Cystatin C
Tested Reactivity Hu
Tested Application ELISA, FLISA, WB
Specificity Cystatin C
Host Mouse
Clonality Monoclonal
Clone Cyst13
Isotype IgG1
Target Name CST3 / Cystatin C
Antigen Species Human
Immunogen Human Cystatin C
Conjugation Un-conjugated
Full Name cystatin C
Alternate Names Cystatin-C; Neuroendocrine basic polypeptide; Post-gamma-globulin; ARMD11; Cystatin-3; Gamma-trace

Application Instructions

Application Suggestion
Tested Application Dilution
ELISAAssay-dependent
FLISA200 ng/ml (Detection Mab)
WBAssay-dependent
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purification with Protein A.
Buffer PBS (pH 7.4) and 0.1% Sodium azide
Preservative 0.1% Sodium azide
Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database links

GeneID: 1471 Human CST3

Swiss-port # P01034 Human Cystatin-C

Gene Symbol CST3
Gene Full Name cystatin C
Background The cystatin superfamily encompasses proteins that contain multiple cystatin-like sequences. Some of the members are active cysteine protease inhibitors, while others have lost or perhaps never acquired this inhibitory activity. There are three inhibitory families in the superfamily, including the type 1 cystatins (stefins), type 2 cystatins and the kininogens. The type 2 cystatin proteins are a class of cysteine proteinase inhibitors found in a variety of human fluids and secretions, where they appear to provide protective functions. The cystatin locus on chromosome 20 contains the majority of the type 2 cystatin genes and pseudogenes. This gene is located in the cystatin locus and encodes the most abundant extracellular inhibitor of cysteine proteases, which is found in high concentrations in biological fluids and is expressed in virtually all organs of the body. A mutation in this gene has been associated with amyloid angiopathy. Expression of this protein in vascular wall smooth muscle cells is severely reduced in both atherosclerotic and aneurysmal aortic lesions, establishing its role in vascular disease. In addition, this protein has been shown to have an antimicrobial function, inhibiting the replication of herpes simplex virus. Alternative splicing results in multiple transcript variants encoding a single protein. [provided by RefSeq, Nov 2014]
Function As an inhibitor of cysteine proteinases, this protein is thought to serve an important physiological role as a local regulator of this enzyme activity. [UniProt]
PTM The Thr-25 variant is O-glycosylated with a core 1 or possibly core 8 glycan. The signal peptide of the O-glycosylated Thr-25 variant is cleaved between Ala-20 and Val-21.

Images (4) Click the Picture to Zoom In

  • ARG10617 anti-CST3 / Cystatin C antibody [Cyst13] WB image

    Western blot: Detection of Human cystatin C after Tricine-SDS-PAGE in reducing conditions. 0.2 µg of Cystatin C purified from Human urine stained with ARG10617 anti-CST3 / Cystatin C antibody [Cyst13].

  • ARG10617 anti-CST3 / Cystatin C antibody [Cyst13] FLISA image

    FLISA: Immunochemical properties of three forms of cystatin C protein, in comparison with antigen from pooled normal Human serum. Cystatin C preparations (at concentration 10 ng/ml) and diluted pooled normal Human serum were analyzed. FLISA was used to measure cystatin C: Capture MAbs: ARG10618 anti-Cystatin C antibody [Cyst24]. Detection MAbs: ARG10617 anti-CST3 / Cystatin C antibody [Cyst13].

  • ARG10617 anti-CST3 / Cystatin C antibody [Cyst13] FLISA image

    FLISA: Calibration curve of the best immunoassays. One-step fluoroimmunoassay in streptavidin coated plates. Capture MAb ARG10618 anti-Cystatin C antibody [Cyst24] is biotinylated (200 ng/well). Detection MAb ARG10617 anti-CST3 / Cystatin C antibody [Cyst13] is Eu3+ - labeled (200 ng/ml).
    Incubation volume 100 µl. time: 30 min at RT.

  • ARG10617 anti-CST3 / Cystatin C antibody [Cyst13] FLISA image

    FLISA: Titration curve of pooled normal Human serum in ARG10618 anti-Cystatin C antibody [Cyst24] - ARG10617 anti-CST3 / Cystatin C antibody [Cyst13] (capture - detection) sandwich fluoroimmunoassays.