ARG45550

anti-CYB5B antibody

anti-CYB5B antibody for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse

Overview

Product Description Rabbit Polyclonal antibody recognizes CYB5B
Tested Reactivity Hu, Ms
Tested Application FACS, ICC/IF, IHC-P, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name CYB5B
Antigen Species Human
Immunogen Recombinant protein containing to human CYB5B.
Conjugation Un-conjugated
Alternate Names CYB5B; cytochrome b5 type B (outer mitochondrial membrane); CYPB5M; CYB5-M; Cytochrome b5 outer mitochondrial membrane isoform; Cytochrome b5 type B; OMB5

Application Instructions

Application Suggestion
Tested Application Dilution
FACS1 - 3 µg/10^6 cells
ICC/IF5 μg/ml
IHC-P2-5 μg/ml
WB0.25-0.5 μg/ml
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Observed Size 20 kDa

Properties

Form Liquid
Purification Affinity purified
Buffer 0.2% Na2HPO4, 0.9% NaCl and 4% Trehalose.
Stabilizer 4% Trehalose
Concentration 0.5 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 66427 Mouse CYB5B

GeneID: 80777 Human CYB5B

Swiss-port # O43169 Human Cytochrome b5 type B

Swiss-port # Q9CQX2 Mouse Cytochrome b5 type B

Gene Symbol CYB5B
Gene Full Name cytochrome b5 type B (outer mitochondrial membrane)
Background Enables heme binding activity. Contributes to nitrite reductase (NO-forming) activity. Involved in nitric oxide biosynthetic process. Located in membrane. Part of nitric-oxide synthase complex. [provided by Alliance of Genome Resources, Feb 2025]
Function Cytochrome b5 is a membrane-bound hemoprotein functioning as an electron carrier for several membrane-bound oxygenases. [UniProt]
Cellular Localization Mitochondrion outer membrane. [UniProt]
Calculated MW 17 kDa
PTM Membrane; Mitochondrion; Mitochondrion outer membrane. [UniProt]

Images (5) Click the Picture to Zoom In

  • ARG45550 anti-CYB5B antibody IHC-P image

    Immunohistochemistry: Human gall bladder adenosquamou stained with ARG45550 anti-CYB5B antibody at 2 μg/ml dilution.

  • ARG45550 anti-CYB5B antibody ICC/IF image

    Immunofluorescence: A549 stained with ARG45550 anti-CYB5B antibody at 5 μg/ml dilution.

  • ARG45550 anti-CYB5B antibody WB image

    Western blot: HepG2 stained with ARG45550 anti-CYB5B antibody at 0.5 μg/ml dilution.

  • ARG45550 anti-CYB5B antibody FACS image

    Flow Cytometry: A549 stained with ARG45550 anti-CYB5B antibody at 1 µg/10^6 cells dilution.

  • ARG45550 anti-CYB5B antibody WB image

    Western blot: Mouse liver stained with ARG45550 anti-CYB5B antibody at 0.5 μg/ml dilution.