anti-CYP3A4 antibody

anti-CYP3A4 antibody for Flow cytometry,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human

Cell Biology and Cellular Response antibody; Metabolism antibody; Signaling Transduction antibody


Product Description Rabbit Polyclonal antibody recognizes CYP3A4
Tested Reactivity Hu
Tested Application FACS, IHC-P, WB
Host Rabbit
Clonality Polyclonal
Target Name CYP3A4
Antigen Species Human
Immunogen KLH-conjugated synthetic peptide corresponding to aa. 228-255 (Center) of Human CYP3A4 (NP_001189784.1).
Conjugation Un-conjugated
Alternate Names EC 1.14.13.-; Nifedipine oxidase; P450PCN1; EC; Taurochenodeoxycholate 6-alpha-hydroxylase; Albendazole sulfoxidase; EC; Cytochrome P450 NF-25; CYPIIIA4; Cytochrome P450 HLp; CYPIIIA3; EC; Cytochrome P450 3A4; Cytochrome P450 3A3; P450C3; EC; Quinine 3-monooxygenase; Albendazole monooxygenase; CP34; CYP3A3; CP33; 1,8-cineole 2-exo-monooxygenase; NF-25; HLP; Cytochrome P450-PCN1; CYP3A

Application Instructions

Application Suggestion
Tested Application Dilution
FACS1:10 - 1:50
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control HepG2


Purification This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Buffer PBS and 0.09% (W/V) Sodium azide
Preservative 0.09% (W/V) Sodium azide
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.


Database Links

GeneID: 1576 Human CYP3A4

Swiss-port # P08684 Human Cytochrome P450 3A4

Gene Symbol CYP3A4
Gene Full Name cytochrome P450, family 3, subfamily A, polypeptide 4
Background This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases that catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and its expression is induced by glucocorticoids and some pharmacological agents. This enzyme is involved in the metabolism of approximately half the drugs in use today, including acetaminophen, codeine, cyclosporin A, diazepam and erythromycin. The enzyme also metabolizes some steroids and carcinogens. This gene is part of a cluster of cytochrome P450 genes on chromosome 7q21.1. Previously another CYP3A gene, CYP3A3, was thought to exist; however, it is now thought that this sequence represents a transcript variant of CYP3A4. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Feb 2011]
Function Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It performs a variety of oxidation reactions (e.g. caffeine 8-oxidation, omeprazole sulphoxidation, midazolam 1'-hydroxylation and midazolam 4- hydroxylation) of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics. Acts as a 1,8-cineole 2- exo-monooxygenase. The enzyme also hydroxylates etoposide. [From Uniprot]
Cellular Localization Endoplasmic reticulum membrane; Single-pass membrane protein. Microsome membrane; Single-pass membrane protein
Research Area Cell Biology and Cellular Response antibody; Metabolism antibody; Signaling Transduction antibody
Calculated MW 57 kDa
PTM Polyubiquitinated in the presence of AMFR and UBE2G1 and also STUB1/CHIP and UBE2D1 (in vitro).

Images (3) Click the Picture to Zoom In

  • ARG54726 anti-CYP3A4 antibody IHC-P image

    Immunohistochemistry: Formalin-fixed and paraffin-embedded Human liver tissue stained with ARG54726 anti-CYP3A4 antibody.

  • ARG54726 anti-CYP3A4 antibody WB image

    Western blot: 35 µg of HepG2 cell lysate stained with ARG54726 anti-CYP3A4 antibody at 1:1000 dilution.

  • ARG54726 anti-CYP3A4 antibody FACS image

    Flow Cytometry: CEM cells stained with ARG54726 anti-CYP3A4 antibody (right histogram) or without primary antibody control (left histogram).