ARG22260

anti-CaMKII alpha antibody [6G9]

anti-CaMKII alpha antibody [6G9] for ELISA,ICC/IF,Immunohistochemistry,Immunoprecipitation,Radioimmunoassay,Western blot and Bovine,Human,Mouse,Rat

publication_link Publication1

Overview

Product Description Mouse Monoclonal antibody [6G9] recognizes CaMKII alpha
Tested Reactivity Hu, Ms, Rat, Bov
Tested Application ELISA, ICC/IF, IHC, IP, RIA, WB
Specificity Detects ~50-60kDa. Recognizes both phosphorylated and non-phosphorylated forms.
Host Mouse
Clonality Monoclonal
Clone 6G9
Isotype IgG1
Target Name CaMKII alpha
Antigen Species Rat
Immunogen Partially purified Rat CaMKII
Conjugation Un-conjugated
Alternate Names CAMKA; CaMK-II subunit alpha; Calcium/calmodulin-dependent protein kinase type II subunit alpha; CaM kinase II subunit alpha; EC 2.7.11.17

Application Instructions

Application Suggestion
Tested Application Dilution
ELISAAssay-dependent
ICC/IF1:50
IHC1:2000
IPAssay-dependent
RIAAssay-dependent
WB1:10000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purification with Protein G.
Buffer PBS (pH 7.4), 0.09% Sodium azide and 50% Glycerol
Preservative 0.09% Sodium azide
Stabilizer 50% Glycerol
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 12322 Mouse CAMK2A

GeneID: 25400 Rat CAMK2A

GeneID: 815 Human CAMK2A

Gene Symbol Camk2a
Gene Full Name calcium/calmodulin-dependent protein kinase II alpha
Background The product of this gene belongs to the serine/threonine protein kinases family, and to the Ca(2+)/calmodulin-dependent protein kinases subfamily. Calcium signaling is crucial for several aspects of plasticity at glutamatergic synapses. This calcium calmodulin-dependent protein kinase is composed of four different chains: alpha, beta, gamma, and delta. The alpha chain encoded by this gene is required for hippocampal long-term potentiation (LTP) and spatial learning. In addition to its calcium-calmodulin (CaM)-dependent activity, this protein can undergo autophosphorylation, resulting in CaM-independent activity. Two transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Nov 2008]
Function CaM-kinase II (CAMK2) is a prominent kinase in the central nervous system that may function in long-term potentiation and neurotransmitter release. Member of the NMDAR signaling complex in excitatory synapses it may regulate NMDAR-dependent potentiation of the AMPAR and synaptic plasticity (By similarity). [UniProt]
Cellular Localization Cytoplasm, Cell Junction, Mitochondrion, Nucleus, Presynaptic Cell Membrane, Synapse
Calculated MW 54 kDa

Images (4) Click the Picture to Zoom In

  • ARG22260 anti-CaMKII alpha antibody [6G9] ICC/IF image

    Immunocytochemistry: Mouse dissociated hippocampal neurons stained with ARG22260 anti-CaMKII alpha antibody [6G9] at 1:1000 dilution.

  • ARG22260 anti-CaMKII alpha antibody [6G9] WB image

    Western blot: Rat brain lysate stained with ARG22260 anti-CaMKII alpha antibody [6G9].

  • ARG22260 anti-CaMKII alpha antibody [6G9] IHC image

    Immunohistochemistry: Formalin fixed colon carcinoma stained with ARG22260 anti-CaMKII alpha antibody [6G9] at 1:10000 dilution (12 hours). Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 200uL for 2 min at RT. Magnification: 40x.

  • ARG22260 anti-CaMKII alpha antibody [6G9] WB image

    Western blot: Rat brain membrane lysate stained with ARG22260 anti-CaMKII alpha antibody [6G9] at 1:1000 dilution.

Specific References

Consuming oxidative frying oil impairs cardiac energy production and calcium recycling, causing cardiac hypertrophy, fibrosis and diastolic dysfunction in male Sprague Dawley rats

WB / Rat

Yu-Shun Lin et al.
J Nutr Biochem.,  (2021)

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