ARG20520

anti-CaMKII phospho (Thr286) antibody [22B1]

anti-CaMKII phospho (Thr286) antibody [22B1] for ELISA,ICC/IF,Immunohistochemistry,Immunoprecipitation,Western blot and Human,Mouse,Rat

Cell Biology and Cellular Response antibody; Neuroscience antibody; Signaling Transduction antibody

Overview

Product Description Mouse Monoclonal antibody [22B1] recognizes CaMKII phospho (Thr286)
Tested Reactivity Hu, Ms, Rat
Tested Application ELISA, ICC/IF, IHC, IP, WB
Specificity Detects phosphorylated CaMKII from rat tissues. Monoclonal antibody 22B1 (anti-phosphoCaMKII is specific for α and β subunits of CaMKII only when they are phosphorylated at Thr-286/287 (in β).
Host Mouse
Clonality Monoclonal
Clone 22B1
Isotype IgG
Target Name CaMKII
Species Rat
Immunogen Phosphospecific peptide around Thr286 of Rat CaMKII (NP_037052.1).
Conjugation Un-conjugated
Full Name calcium/calmodulin-dependent protein kinase II alpha
Alternate Names CAMKA; CaMK-II subunit alpha; Calcium/calmodulin-dependent protein kinase type II subunit alpha; CaM kinase II subunit alpha; EC 2.7.11.17

Application Instructions

Application Suggestion
Tested Application Dilution
ELISAAssay-dependent
ICC/IF1:1000
IHC1:100
IPAssay-dependent
WB1:1000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control Rat brain tissue extract.
Calculated MW 54 kDa
Observed Size ~50 kDa

Properties

Form Liquid
Purification Protein G purified.
Buffer PBS (pH 7.4), 0.09% Sodium azide and 50% Glycerol
Preservative 0.09% Sodium azide
Stabilizer 50% Glycerol
Concentration 1 mg/ml
Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database links

GeneID: 12322 Mouse CAMK2A

GeneID: 25400 Rat CAMK2A

GeneID: 815 Human CAMK2A

Gene Symbol Camk2a
Gene Full Name calcium/calmodulin-dependent protein kinase II alpha
Background The product of this gene belongs to the serine/threonine protein kinases family, and to the Ca(2+)/calmodulin-dependent protein kinases subfamily. Calcium signaling is crucial for several aspects of plasticity at glutamatergic synapses. This calcium calmodulin-dependent protein kinase is composed of four different chains: alpha, beta, gamma, and delta. The alpha chain encoded by this gene is required for hippocampal long-term potentiation (LTP) and spatial learning. In addition to its calcium-calmodulin (CaM)-dependent activity, this protein can undergo autophosphorylation, resulting in CaM-independent activity. Two transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Nov 2008]
Function CaM-kinase II (CAMK2) is a prominent kinase in the central nervous system that may function in long-term potentiation and neurotransmitter release. Member of the NMDAR signaling complex in excitatory synapses it may regulate NMDAR-dependent potentiation of the AMPAR and synaptic plasticity. [UniProt]
Research Area Cell Biology and Cellular Response antibody; Neuroscience antibody; Signaling Transduction antibody

Images (5) Click the Picture to Zoom In

  • ARG20520 anti-CaMKII phospho (Thr286) antibody [22B1] IHC-P image

    Immunohistochemistry: Bouin's fixed paraffin-embedded backskin sections of transgenic mice- muscle, and hair follicle stained with ARG20520 anti-CaMKII phospho (Thr286) antibody [22B1].

  • ARG20520 anti-CaMKII phospho (Thr286) antibody [22B1] WB image

    Western blot: Rat brain lysate stained with ARG20520 anti-CaMKII phospho (Thr286) antibody [22B1].

  • ARG20520 anti-CaMKII phospho (Thr286) antibody [22B1] ICC/IF image

    Immunofluorescence: Rat hippocampal neurons stained with ARG20520 anti-CaMKII phospho (Thr286) antibody [22B1].

  • ARG20520 anti-CaMKII phospho (Thr286) antibody [22B1] IHC image

    Immunohistochemistry: Formalin-fixed Human colon carcinoma stained with ARG20520 anti-CaMKII phospho (Thr286) antibody [22B1] at 1:5000 for 12 hours at 4°C. Secondary antibody: Biotin Goat anti-Mouse at 1:2000 for 1 hour at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 200 µl for 2 min at RT. Magnification: 40x.

  • ARG20520 anti-CaMKII phospho (Thr286) antibody [22B1] WB image

    Western blot: 1) Ventricles lysate from 14-Day-Old normal Mouse, 2) Ventricles lysate from 14-Day-Old CaM overexpressing transgenic Mouse line 2295, 3) Ventricles lysate from 14-Day-Old CaM-8 (mutant CaM) expressing transgenic Mouse line 4466, stained with ARG20520 anti-CaMKII phospho (Thr286) antibody [22B1]. (PMID: 10935538).

Clone References

CaMKII, but not protein kinase A, regulates Rpt6 phosphorylation and proteasome activity during the formation of long-term memories.

WB / Rat

Jarome TJ et al.
Front Behav Neurosci.,  (2013)

publication_link

 

hr_line

Chronic caffeine treatment prevents stress-induced LTP impairment: the critical role of phosphorylated CaMKII and BDNF.

WB / Rat

Alzoubi KH et al.
J Mol Neurosci.,  (2013)

publication_link

 

hr_line

The effects of oral taurine administration on behavior and hippocampal signal transduction in rats.

WB / Rat

Iio W et al.
Amino Acids.,  (2012)

publication_link

 

hr_line

CaMKII-dependent diastolic SR Ca2+ leak and elevated diastolic Ca2+ levels in right atrial myocardium of patients with atrial fibrillation.

WB / Human

Neef S et al.
Circ Res.,  (2010)

publication_link

 

hr_line

Rapid, bidirectional remodeling of synaptic NMDA receptor subunit composition by A-type K+ channel activity in hippocampal CA1 pyramidal neurons.

ICC/IF / Rat

Jung SC et al.
Neuron.,  (2008)

publication_link

 

hr_line