anti-CaMKII phospho (Thr286) antibody

anti-CaMKII phospho (Thr286) antibody for Western blot and Rat

Cell Biology and Cellular Response antibody; Neuroscience antibody; Signaling Transduction antibody


Product Description Rabbit Polyclonal antibody recognizes CaMKII phospho (Thr286)
Tested Reactivity Rat
Predict Reactivity Hu, Ms, Xenopus laevis
Tested Application WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name CaMKII
Antigen Species Rat
Immunogen Synthetic phospho-peptide corresponding to amino acid residues surrounding Thr286 conjugated to KLH
Conjugation Un-conjugated
Alternate Names CAMKA; CaMK-II subunit alpha; Calcium/calmodulin-dependent protein kinase type II subunit alpha; CaM kinase II subunit alpha; EC

Application Instructions

Application Suggestion
Tested Application Dilution
Application Note Specific for the ~50k α-CaM Kinase II and the ~60k β-CaM Kinase II proteins phosphorylated at Thr286. Immunolabeling is blocked by the λ-phosphatase treatment.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.


Form Liquid
Purification Affinity Purified
Buffer 10 mM HEPES (pH 7.5), 150 mM NaCl, 0.1 mg/ml BSA and 50% Glycerol
Stabilizer 0.1 mg/ml BSA, 50% Glycerol
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.


Database Links

GeneID: 25400 Rat CAMK2A

Swiss-port # P11275 Rat Calcium/calmodulin-dependent protein kinase type II subunit alpha

Gene Symbol CAMK2A/B
Gene Full Name calcium/calmodulin-dependent protein kinase II alpha
Background Ca2+/calmodulin-dependent protein kinase II (CaM Kinase II) is a multi-functional calcium and calmodulin-dependent protein kinase that mediates cellular responses to a wide variety of intercellular signals (Kennedy, 1998; Schulman and Hanson, 1993). CaM Kinase II has been shown to regulate diverse cellular functions including synaptic plasticity, neurotransmitter synthesis and release, gene expression, ion channel function, carbohydrate metabolism, cytoskeletal function, and Ca2+-homeostasis (Gleason et al., 2003; Soderling, 2000; Hudmon and Schulman, 2002). Phosphorylation of Thr286 on the kinase produces an autonomously active form of CaM Kinase II (Meng et al., 2003; Picciotto et al., 1993). Autophosphorylation of Thr305 inhibits the activity CaM Kinase II. Phosphorylation at this site appears to reduce the association of CaM Kinase II with the PSD and reduce LTP and learning (Elgersma et al., 2002).
Research Area Cell Biology and Cellular Response antibody; Neuroscience antibody; Signaling Transduction antibody
Calculated MW 54 kDa

Images (1) Click the Picture to Zoom In

  • ARG52238 anti-CaMKII phospho (Thr286) antibody WB image

    Western blot: Rat cortex lysate showing phospho-specific immunolabeling of the ~50 kDa alpha-subunit and ~60 kDa beta-subunit of the CaM Kinase II protein phosphorylated at Thr 286 stained with ARG52238 anti-CaMKII phospho (Thr286) antibody.