ARG11111

anti-Calbindin antibody

anti-Calbindin antibody for Western blot,ICC/IF,IHC-Frozen sections and Human,Mouse,Rat,Cow,Pig

Overview

Product Description Chicken Polyclonal antibody recognizes Calbindin
Tested Reactivity Hu, Ms, Rat, Cow, Pig
Tested Application ICC/IF, IHC-Fr, WB
Host Chicken
Clonality Polyclonal
Isotype IgY
Target Name Calbindin
Antigen Species Human
Immunogen Recombinant full-length Human Calbindin.
Conjugation Un-conjugated
Alternate Names Vitamin D-dependent calcium-binding protein, avian-type; Calbindin; CALB; Calbindin D28; D-28K

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IF1:1000 - 1:5000
IHC-Fr1:1000 - 1:5000
WB1:5000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Observed Size 30 or 26 kDa

Properties

Form Liquid
Buffer PBS and 5 mM Sodium azide.
Preservative 5 mM Sodium azide
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 12307 Mouse CALB1

GeneID: 793 Human CALB1

GeneID: 83839 Rat CALB1

Gene Symbol CALB1
Gene Full Name calbindin 1, 28kDa
Background The protein encoded by this gene is a member of the calcium-binding protein superfamily that includes calmodulin and troponin C. Originally described as a 27 kDa protein, it is now known to be a 28 kDa protein. It contains four active calcium-binding domains, and has two modified domains that are thought to have lost their calcium binding capability. This protein is thought to buffer entry of calcium upon stimulation of glutamate receptors. Depletion of this protein was noted in patients with Huntington disease. [provided by RefSeq, Jan 2015]
Function Buffers cytosolic calcium. May stimulate a membrane Ca(2+)-ATPase and a 3',5'-cyclic nucleotide phosphodiesterase. [UniProt]
Calculated MW 30 kDa

Images (3) Click the Picture to Zoom In

  • ARG11111 anti-Calbindin antibody IHC-Fr image

    Immunohistochemistry: Frozen section of Rat cerebellum tissue stained with ARG11111 anti-Calbindin antibody (green) at 1:2000 dilution, and costained with ARG10737 anti-MeCP2 antibody (red) at 1:5000 dilution. (Sample preparation: Following transcardial perfusion with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45 µM, and free-floating sections were stained with above antibodies.).

  • ARG11111 anti-Calbindin antibody WB image

    Western blot: Rat cerebellum, Pig hippocampus and Cow cerebellum lysates stained with ARG11111 anti-Calbindin antibody at 1:5000 dilution.

  • ARG11111 anti-Calbindin antibody WB image

    Western blot: Full length recombinant Human parvalbumin, calretinin and calbindin proteins stained with ARG11111 anti-Calbindin antibody at 1:5000 dilution.