ARG40287

anti-Calbindin antibody

anti-Calbindin antibody for IHC-Formalin-fixed paraffin-embedded sections,IHC-Frozen sections,Western blot and Human,Mouse,Rat

Overview

Product Description Goat Polyclonal antibody recognizes Calbindin
Tested Reactivity Hu, Ms, Rat
Predict Reactivity Cow, Dog, Pig
Tested Application IHC-Fr, IHC-P, WB
Host Goat
Clonality Polyclonal
Isotype IgG
Target Name Calbindin
Antigen Species Human
Immunogen Synthetic peptide around the internal region of Human Calbindin. (NP_004920.1. C-KTFVDQYGQRDDGK)
Conjugation Un-conjugated
Alternate Names Vitamin D-dependent calcium-binding protein, avian-type; Calbindin; CALB; Calbindin D28; D-28K

Application Instructions

Application Suggestion
Tested Application Dilution
IHC-Fr0.1 - 0.3 µg/ml
IHC-P5 µg/ml
WB0.03 - 0.1 µg/ml
Application Note WB: Recommend incubate at RT for 1h.
IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0) at 80°C for 30 min.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Observed Size ~ 26 kDa

Properties

Form Liquid
Purification Ammonium sulphate precipitation followed by affinity purification with immunogen.
Buffer Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA.
Preservative 0.02% Sodium azide
Stabilizer 0.5% BSA
Concentration 0.5 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 12307 Mouse CALB1

GeneID: 793 Human CALB1

GeneID: 83839 Rat CALB1

Gene Symbol CALB1
Gene Full Name calbindin 1, 28kDa
Background The protein encoded by this gene is a member of the calcium-binding protein superfamily that includes calmodulin and troponin C. Originally described as a 27 kDa protein, it is now known to be a 28 kDa protein. It contains four active calcium-binding domains, and has two modified domains that are thought to have lost their calcium binding capability. This protein is thought to buffer entry of calcium upon stimulation of glutamate receptors. Depletion of this protein was noted in patients with Huntington disease. [provided by RefSeq, Jan 2015]
Function Buffers cytosolic calcium. May stimulate a membrane Ca(2+)-ATPase and a 3',5'-cyclic nucleotide phosphodiesterase. [UniProt]
Calculated MW 30 kDa

Images (4) Click the Picture to Zoom In

  • ARG40287 anti-Calbindin antibody IHC-Fr image

    Immunohistochemistry: PFA-perfused cryosection of Human hypothalamus tissue stained with ARG40287 anti-Calbindin antibody at 0.1 µg/ml dilution.

  • ARG40287 anti-Calbindin antibody WB image

    Western blot: 35 µg of Human cerebellum lysate (in RIPA buffer) stained with ARG40287 anti-Calbindin antibody at 0.03 µg/ml dilution and incubated at RT for 1 hour.

  • ARG40287 anti-Calbindin antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Human kidney stained with ARG40287 anti-Calbindin antibody at 5 µg/ml dilution. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0).

  • ARG40287 anti-Calbindin antibody WB image

    Western blot: 35 µg of Mouse brain and Rat brain lysates (in RIPA buffer) stained with ARG40287 anti-Calbindin antibody at 0.1 µg/ml dilution and incubated at RT for 1 hour.