ARG40276

anti-Calbindin antibody [CB-955]

anti-Calbindin antibody [CB-955] for Western blot,IHC-Formalin-fixed paraffin-embedded sections,IHC-Frozen sections and Human,Mouse,Rat

Overview

Product Description Mouse Monoclonal antibody [CB-955] recognizes Calbindin
Tested Reactivity Hu, Ms, Rat
Predict Reactivity Bov, Mk
Tested Application IHC-Fr, IHC-P, WB
Host Mouse
Clonality Monoclonal
Clone CB-955
Isotype IgG1
Target Name Calbindin
Antigen Species Bovine
Immunogen Bovine kidney Calbindin.
Conjugation Un-conjugated
Alternate Names Vitamin D-dependent calcium-binding protein, avian-type; Calbindin; CALB; Calbindin D28; D-28K

Application Instructions

Application Suggestion
Tested Application Dilution
IHC-FrAssay-dependent
IHC-P1 - 2 µg/ml
WB0.5 - 1 µg/ml
Application Note IHC-P: Antigen Retrieval: By heat mediation.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Observed Size ~ 28 kDa

Properties

Form Liquid
Purification Unpurified.
Buffer Mouse ascites fluid, 1.2% Sodium acetate, 0.01% Sodium azide and 2% BSA.
Preservative 0.01% Sodium azide
Stabilizer 2% BSA
Concentration 0.1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 12307 Mouse CALB1

GeneID: 793 Human CALB1

GeneID: 83839 Rat CALB1

Gene Symbol CALB1
Gene Full Name calbindin 1, 28kDa
Background The protein encoded by this gene is a member of the calcium-binding protein superfamily that includes calmodulin and troponin C. Originally described as a 27 kDa protein, it is now known to be a 28 kDa protein. It contains four active calcium-binding domains, and has two modified domains that are thought to have lost their calcium binding capability. This protein is thought to buffer entry of calcium upon stimulation of glutamate receptors. Depletion of this protein was noted in patients with Huntington disease. [provided by RefSeq, Jan 2015]
Function Buffers cytosolic calcium. May stimulate a membrane Ca(2+)-ATPase and a 3',5'-cyclic nucleotide phosphodiesterase. [UniProt]
Calculated MW 30 kDa

Images (5) Click the Picture to Zoom In

  • ARG40276 anti-Calbindin antibody [CB-955] IHC-P image

    Immunohistochemistry: Paraffin-embedded Mouse kidney stained with ARG40276 anti-Calbindin antibody [CB-955].

  • ARG40276 anti-Calbindin antibody [CB-955] WB image

    Western blot: Rat brain (lane 1 and 2), Rat kidney (lane 3) and Rat lung (lane 4) tissue lysates stained with ARG40276 anti-Calbindin antibody [CB-955].

  • ARG40276 anti-Calbindin antibody [CB-955] IHC-P image

    Immunohistochemistry: Paraffin-embedded Rat kidney stained with ARG40276 anti-Calbindin antibody [CB-955].

  • ARG40276 anti-Calbindin antibody [CB-955] IHC-P image

    Immunohistochemistry: Paraffin-embedded Human intestinal cancer stained with ARG40276 anti-Calbindin antibody [CB-955].

  • ARG40276 anti-Calbindin antibody [CB-955] IHC-P image

    Immunohistochemistry: Paraffin-embedded Human mammary cancer stained with ARG40276 anti-Calbindin antibody [CB-955].