anti-Calpastatin antibody [CSL1 5]

anti-Calpastatin antibody [CSL1 5] for ELISA,Western blot,Immunohistochemistry and Human

Cell Biology and Cellular Response antibody; Neuroscience antibody


Product Description Mouse Monoclonal antibody [CSL1 5] recognizes Calpastatin
Tested Reactivity Hu
Tested Application ELISA, IHC, WB
Specificity This antibody specifically recognizes an epitope within domain III of human calpastatin.
Host Mouse
Clonality Monoclonal
Clone CSL1 5
Isotype IgG1
Target Name Calpastatin
Antigen Species Human
Immunogen Recombinant human muscle-type calpastatin.
Conjugation Un-conjugated
Alternate Names Calpastatin; PLACK; Calpain inhibitor; Sperm BS-17 component; BS-17

Application Instructions

Application Note ELISA: 1 - 10 ug/ml with immobilized antigen.
Western blot: 1 - 10 ug/ml, denaturing and non-denaturing conditions.
Immunohistochemistry: 1 - 10 ug/ml, frozen tissue sections.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.


Form Liquid
Buffer 10 mM PBS (pH 7.4) and 1% BSA
Stabilizer 1% BSA
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.


Database Links

GeneID: 831 Human CAST

Swiss-port # P20810 Human Calpastatin

Gene Symbol CAST
Gene Full Name calpastatin
Background The protein encoded by this gene is an endogenous calpain (calcium-dependent cysteine protease) inhibitor. It consists of an N-terminal domain L and four repetitive calpain-inhibition domains (domains 1-4), and it is involved in the proteolysis of amyloid precursor protein. The calpain/calpastatin system is involved in numerous membrane fusion events, such as neural vesicle exocytosis and platelet and red-cell aggregation. The encoded protein is also thought to affect the expression levels of genes encoding structural or regulatory proteins. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Jun 2010]
Function Specific inhibition of calpain (calcium-dependent cysteine protease). Plays a key role in postmortem tenderization of meat and have been proposed to be involved in muscle protein degradation in living tissue. [UniProt]
Research Area Cell Biology and Cellular Response antibody; Neuroscience antibody
Calculated MW 77 kDa
PTM The N-terminus is blocked.