ARG10703

anti-Calretinin antibody [6A9]

anti-Calretinin antibody [6A9] for ICC/IF,IHC-Frozen sections,Western blot and Human,Mouse,Rat,Cow

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anti-Calretinin antibody [6A9]

Mouse Monoclonal antibody [6A9] recognizes Calretinin

ARG10703 Arigo biolaboratories
5.0 out of 5 based on 2
anti-Calretinin antibody [6A9] is rated 5.0 out of 5 by 2.

anti-Calretinin antibody [6A9]

Overview

Product Description

Mouse Monoclonal antibody [6A9] recognizes Calretinin

Tested Reactivity Hu, Ms, Rat, Cow
Tested Application ICC/IF, IHC-Fr, WB
Host Mouse
Clonality Monoclonal
Clone 6A9
Isotype IgA
Target Name Calretinin
Antigen Species Human
Immunogen Full-length recombinant Human protein.
Conjugation Un-conjugated
Full Name calbindin 2
Alternate Names CAB29; CR; CAL2; 29 kDa calbindin; Calretinin

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IF1:2000 - 1:5000
IHC-Fr1:2000 - 1:5000
WB1:2000 - 1:5000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Affinity purification.
Buffer PBS, 5 mM Sodium azide and 50% Glycerol.
Preservative 5 mM Sodium azide
Stabilizer 50% Glycerol
Concentration 1 mg/ml
Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database links

GeneID: 117059 Rat CALB2

GeneID: 12308 Mouse CALB2

GeneID: 794 Human CALB2

Gene Symbol CALB2
Gene Full Name calbindin 2
Background This gene encodes an intracellular calcium-binding protein belonging to the troponin C superfamily. Members of this protein family have six EF-hand domains which bind calcium. This protein plays a role in diverse cellular functions, including message targeting and intracellular calcium buffering. It also functions as a modulator of neuronal excitability, and is a diagnostic marker for some human diseases, including Hirschsprung disease and some cancers. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2010]
Function Calretinin is a calcium-binding protein which is abundant in auditory neurons. [UniProt]

Images (7) Click the Picture to Zoom In

  • ARG10703 anti-Calretinin antibody [6A9] WB image

    Western blot: 15 µg of Rat brain lysate stained with ARG10703 anti-Calretinin antibody [6A9] at 1:2500 dilution.

  • ARG10703 anti-Calretinin antibody [6A9] ICC/IF image

    Immunocytochemistry: Rat mixed neuron / glial cultures stained with ARG10703 anti-Calretinin antibody [6A9] (red) at 1:2000, and co-stained with chicken polyclonal antibody to Vimentin (green). Calretinin is prominently expressed in small number of interneurons, while astrocytes and fibroblasts were visualized with the vimentin antibody.

  • ARG10703 anti-Calretinin antibody [6A9] IHC-Fr image

    Immunohistochemistry: Frozen section of Rat hippocampus stained with ARG10703 anti-Calretinin antibody [6A9] (green) at 1:2000 dilution and costained with ARG10686 anti-Parvalbumin antibody (red) at 1:1000 dilution. DAPI (blue) for nuclear staining. (Sample preparation: Following transcardial perfusion of Rat with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45 µM, and free-floating sections were stained with above antibodies.)

  • ARG10703 anti-Calretinin antibody [6A9] IHC-Fr image

    Immunohistochemistry: Frozen sections of adult Rat cortical (45 µM; fixed by transcardial perfusion with 4% paraformaldehyde) was stained with ARG10703 anti-Calretinin antibody [6A9] at 1:1000 (red) and co-stained with chicken polyclonal antibody to calbinidin (green). In the motor cortex, calretinin is expressed in a small population of interneurons that do not express calbindin. Because each antibody specifically labels a different population of cells exclusively, the cells are either stained with red, or green.

  • ARG10703 anti-Calretinin antibody [6A9] WB image

    Western blot: Rat brain, pavalbumin, calretinin, and calbindin recombinant proteins was stained with ARG10703 anti-Calretinin antibody [6A9] at 1:5000 dilution.

  • ARG10703 anti-Calretinin antibody [6A9] WB image

    Western blot: Rat brain, Rat spinal cord, Mouse brain, Mouse spinal cord and Cow spinal cord lysates stained with ARG10703 anti-Calretinin antibody [6A9] (green) at 1:2000 dilution.

  • ARG10703 anti-Calretinin antibody [6A9] IHC-Fr image

    Immunohistochemistry: Frozen sections of adult Mouse brain hippocampal (45 µM; fixed by transcardial perfusion with 4% paraformaldehyde) was stained with ARG10703 anti-Calretinin antibody [6A9] at 1:1000 (red) and co-stained with antibody to calbinidin (green). In the stratum radiatum of CA1 region, calretinin expresses in a small number of interneurons that do not express calbindin. As a result, our antibodies label different neurons in either red or green. Insets are high-magnification images of the boxed area in each picture. Blue is a hoechst DNA staining.

Customer's Feedback

nuts_pic      Excellent

Review for anti-Calretinin antibody [6A9]

Application:WB

Sample:Rat brain

Sample Loading Amount:15 µg

Primary Antibody Dilution Factor:1:2500

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC

nuts_pic      Excellent

Review for anti-Calretinin antibody [6A9]

Application:WB

Sample:Mouse Cerebellum

Sample Loading Amount:15 µg

Primary Antibody Dilution Factor:1:2500

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC