anti-Cardiac Troponin I antibody [4C2]
anti-Cardiac Troponin I antibody [4C2] for ELISA,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Bovine,Dog,Human,Mouse,Pig,Rat
Cell Biology and Cellular Response antibody; Controls and Markers antibody; Developmental Biology antibody; Signaling Transduction antibody
|Product Description||Mouse Monoclonal antibody [4C2] recognizes Cardiac Troponin I|
|Tested Reactivity||Hu, Ms, Rat, Bov, Dog, Pig|
|Tested Application||ELISA, IHC-P, WB|
|Target Name||Cardiac Troponin I|
|Immunogen||free human cTnI, native human cTn complex, or human cTnI peptides.|
|Epitope||a.a. 23-29 (SSNYRAYA)|
|Alternate Names||RCM1; cTnI; Cardiac troponin I; TNNC1; CMD1FF; CMD2A; Troponin I, cardiac muscle; CMH7|
|Application Note||ELISA: This antibody (in biotinylated form) can be used as capture antibody in sandwich ELISA in combination with Eu-labeled ARG10125 anti-Cardiac Troponin I antibody [19C7] as detection antibody.
IHC-P: Antigen Retrieval: Heat mediated was performed using Tris/EDTA buffer pH 9.0 or citrate buffer pH 6.0.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
|Purification||Protein A affinity purified.|
|Buffer||PBS (pH 7.4) and 0.1% Sodium azide|
|Preservative||0.1% Sodium azide|
|Storage Instruction||For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.|
|Note||For laboratory research only, not for drug, diagnostic or other use.|
|Gene Full Name||troponin I type 3 (cardiac)|
|Background||Troponin I (TnI), along with troponin T (TnT) and troponin C (TnC), is one of 3 subunits that form the troponin complex of the thin filaments of striated muscle. TnI is the inhibitory subunit; blocking actin-myosin interactions and thereby mediating striated muscle relaxation. The TnI subfamily contains three genes: TnI-skeletal-fast-twitch, TnI-skeletal-slow-twitch, and TnI-cardiac. This gene encodes the TnI-cardiac protein and is exclusively expressed in cardiac muscle tissues. Mutations in this gene cause familial hypertrophic cardiomyopathy type 7 (CMH7) and familial restrictive cardiomyopathy (RCM). [provided by RefSeq, Jul 2008]|
|Function||Troponin I is the inhibitory subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity. [UniProt]|
|Research Area||Cell Biology and Cellular Response antibody; Controls and Markers antibody; Developmental Biology antibody; Signaling Transduction antibody|
|Calculated MW||24 kDa|
|PTM||Phosphorylated at Ser-42 and Ser-44 by PRKCE; phosphorylation increases myocardium contractile dysfunction (By similarity). Phosphorylated at Ser-23 and Ser-24 by PRKD1; phosphorylation reduces myofilament calcium sensitivity. Phosphorylated preferentially at Thr-31. Phosphorylation by STK4/MST1 alters its binding affinity to TNNC1 (cardiac Tn-C) and TNNT2 (cardiac Tn-T).|
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ARG10144 anti-Cardiac Troponin I antibody [4C2] ELISA image
Sandwich ELISA: Calibration curve for purified Rat Cardiac Troponin I. ARG10144 anti-Cardiac Troponin I antibody [4C2] (in biotinylated form) can be used as capture antibody in sandwich ELISA in combination with Eu-labeled ARG10125 anti-Cardiac Troponin I antibody [19C7] as detection antibody.
Loading: 200 ng/well of capture and detection antibodies.