ARG10144

anti-Cardiac Troponin I antibody [4C2]

anti-Cardiac Troponin I antibody [4C2] for Western blot and Human,Rat,Mouse,Dog,Bovine,Pig

Cell Biology and Cellular Response antibody; Controls and Markers antibody; Developmental Biology antibody; Signaling Transduction antibody

Overview

Product Description Mouse Monoclonal antibody [4C2] recognizes Cardiac Troponin I
Tested Reactivity Hu, Rat, Ms, Dog, Bov, Pig
Tested Application WB
Host Mouse
Clonality Monoclonal
Clone 4C2
Isotype IgG2a
Target Name Cardiac Troponin I
Antigen Species Human
Immunogen free human cTnI, native human cTn complex, or human cTnI peptides.
Conjugation Un-conjugated
Full Name troponin I type 3 (cardiac)
Alternate Names RCM1; cTnI; Cardiac troponin I; TNNC1; CMD1FF; CMD2A; Troponin I, cardiac muscle; CMH7

Application Instructions

Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Protein A affinity purified.
Buffer PBS (pH 7.4) and 0.1% Sodium azide
Preservative 0.1% Sodium azide
Concentration 1.0-2.0 mg/ml
Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database links

GeneID: 21954 Mouse TNNI3

GeneID: 29248 Rat TNNI3

GeneID: 511094 Bovine TNNI3

Gene Symbol TNNI3
Background Troponin I (TnI), along with troponin T (TnT) and troponin C (TnC), is one of 3 subunits that form the troponin complex of the thin filaments of striated muscle. TnI is the inhibitory subunit; blocking actin-myosin interactions and thereby mediating striated muscle relaxation. The TnI subfamily contains three genes: TnI-skeletal-fast-twitch, TnI-skeletal-slow-twitch, and TnI-cardiac. This gene encodes the TnI-cardiac protein and is exclusively expressed in cardiac muscle tissues. Mutations in this gene cause familial hypertrophic cardiomyopathy type 7 (CMH7) and familial restrictive cardiomyopathy (RCM). [provided by RefSeq, Jul 2008]
Function Troponin I is the inhibitory subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity. [UniProt]
Resrarch Area Cell Biology and Cellular Response antibody; Controls and Markers antibody; Developmental Biology antibody; Signaling Transduction antibody
PTM Phosphorylated at Ser-42 and Ser-44 by PRKCE; phosphorylation increases myocardium contractile dysfunction (By similarity). Phosphorylated at Ser-23 and Ser-24 by PRKD1; phosphorylation reduces myofilament calcium sensitivity. Phosphorylated preferentially at Thr-31. Phosphorylation by STK4/MST1 alters its binding affinity to TNNC1 (cardiac Tn-C) and TNNT2 (cardiac Tn-T).

Clone References

Molecular beacon-enabled purification of living cells by targeting cell type-specific mRNAs.

Wile BM et al.
Nat Protoc.,  (2014)

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Myosin cross-bridge dynamics in patients with hypertension and concentric left ventricular remodeling.

WB / 

Donaldson C et al.
Circ Heart Fail.,  (2012)

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Free antibody sample