ARG67294
anti-Cardiac Troponin I phospho (Ser23/Ser24) antibody
anti-Cardiac Troponin I phospho (Ser23/Ser24) antibody for Western blot and Human,Mouse,Rat
Cell Biology and Cellular Response antibody; Controls and Markers antibody; Developmental Biology antibody; Signaling Transduction antibody
Overview
| Product Description | Rabbit Polyclonal antibody recognizes Cardiac Troponin I phospho (Ser23/Ser24) |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Target Name | Cardiac Troponin I |
| Immunogen | KLH-conjugated phospho-peptide |
| Conjugation | Un-conjugated |
| Alternate Names | RCM1; cTnI; Cardiac troponin I; TNNC1; CMD1FF; CMD2A; Troponin I, cardiac muscle; CMH7 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||
| Observed Size | 24 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity chromatography purified |
| Buffer | 0.42% Potassium phosphate (pH 7.3), 0.87% NaCl, 0.01% Sodium azide and 30% Glycerol. |
| Preservative | 0.01% Sodium azide |
| Stabilizer | 30 % Glycerol |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | TNNI3 |
| Gene Full Name | troponin I type 3 (cardiac) |
| Background | Troponin I (TnI), along with troponin T (TnT) and troponin C (TnC), is one of 3 subunits that form the troponin complex of the thin filaments of striated muscle. TnI is the inhibitory subunit; blocking actin-myosin interactions and thereby mediating striated muscle relaxation. The TnI subfamily contains three genes: TnI-skeletal-fast-twitch, TnI-skeletal-slow-twitch, and TnI-cardiac. This gene encodes the TnI-cardiac protein and is exclusively expressed in cardiac muscle tissues. Mutations in this gene cause familial hypertrophic cardiomyopathy type 7 (CMH7) and familial restrictive cardiomyopathy (RCM). [provided by RefSeq, Jul 2008] |
| Function | Troponin I is the inhibitory subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity. [UniProt] |
| Research Area | Cell Biology and Cellular Response antibody; Controls and Markers antibody; Developmental Biology antibody; Signaling Transduction antibody |
| Calculated MW | 24 kDa |
| PTM | Phosphorylated at Ser-42 and Ser-44 by PRKCE; phosphorylation increases myocardium contractile dysfunction (By similarity). Phosphorylated at Ser-23 and Ser-24 by PRKD1; phosphorylation reduces myofilament calcium sensitivity. Phosphorylated preferentially at Thr-31. Phosphorylation by STK4/MST1 alters its binding affinity to TNNC1 (cardiac Tn-C) and TNNT2 (cardiac Tn-T). |
Images (3) Click the Picture to Zoom In
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ARG67294 anti-Cardiac Troponin I phospho (Ser23/Ser24) antibody WB image
Western blot: A549 stained with ARG67294 anti-Cardiac Troponin I phospho (Ser23/Ser24) antibody.
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ARG67294 anti-Cardiac Troponin I phospho (Ser23/Ser24) antibody WB image
Western blot: Rat heart stained with ARG67294 anti-Cardiac Troponin I phospho (Ser23/Ser24) antibody.
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ARG67294 anti-Cardiac Troponin I phospho (Ser23/Ser24) antibody WB image
Western blot: Mouse testis stained with ARG67294 anti-Cardiac Troponin I phospho (Ser23/Ser24) antibody.
