anti-Cardiac Troponin I phospho (Ser43) antibody

anti-Cardiac Troponin I phospho (Ser43) antibody for Western blot and Mouse,Rat

Cell Biology and Cellular Response antibody; Controls and Markers antibody; Developmental Biology antibody; Signaling Transduction antibody


Product Description Rabbit Polyclonal antibody recognizes Cardiac Troponin I phospho (Ser43)
Tested Reactivity Ms, Rat
Predict Reactivity Hu, Bov, Dog, NHuPrm, Sheep, Xenopus laevis, Zfsh
Tested Application WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name Cardiac Troponin I
Antigen Species Mouse
Immunogen Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser43 conjugated to KLH
Conjugation Un-conjugated
Alternate Names RCM1; cTnI; Cardiac troponin I; TNNC1; CMD1FF; CMD2A; Troponin I, cardiac muscle; CMH7

Application Instructions

Application Suggestion
Tested Application Dilution
Application Note Specific for the ~25k cardiac troponin I protein phosphorylated at Ser43. Immunolabeling is greatly decreased with lambda-phosphatase treatment.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.


Form Liquid
Purification Affinity Purified
Buffer 10 mM HEPES (pH 7.5), 150 mM NaCl, 0.1 mg/ml BSA and 50% Glycerol
Stabilizer 0.1 mg/ml BSA, 50% Glycerol
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.


Database Links

GeneID: 21954 Mouse TNNI3

GeneID: 29248 Rat TNNI3

Swiss-port # P23693 Rat Troponin I, cardiac muscle

Swiss-port # P48787 Mouse Troponin I, cardiac muscle

Gene Symbol TNNI3
Gene Full Name troponin I, cardiac 3
Background Troponin I (TnI) is 1 of 3 subunits, along with troponin C (TnC) and Troponin T (TnT) of troponin complex found in cardiac (cTnI) and fast skeletal (fsTnI) muscle (Noland et al, 1995). Protein kinase C-mediated phosphorylation of cardiac myofilament proteins has been shown to depress the actomyosin interaction and may be important during heart failure. (Montgomery et al, 2002) Studies indicate that phosphorylation of Ser43 and Ser45 of cardiac troponin I plays a substantial role in the PKCmediated depression. (Montgomery et al, 2002).
Research Area Cell Biology and Cellular Response antibody; Controls and Markers antibody; Developmental Biology antibody; Signaling Transduction antibody
Calculated MW 24 kDa
PTM Phosphorylated at Ser-42 and Ser-44 by PRKCE; phosphorylation increases myocardium contractile dysfunction (By similarity). Phosphorylated at Ser-23 and Ser-24 by PRKD1; phosphorylation reduces myofilament calcium sensitivity. Phosphorylated preferentially at Thr-31. Phosphorylation by STK4/MST1 alters its binding affinity to TNNC1 (cardiac Tn-C) and TNNT2 (cardiac Tn-T).

Images (1) Click the Picture to Zoom In

  • ARG52455 anti-Cardiac Troponin I phospho (Ser43) antibody WB image

    Western blot: Mouse heart lysate showing specific immunolabeling of the ~25k cTnI protein phosphorylated at Ser 43 (control) stained with ARG52455 anti-Cardiac Troponin I phospho (Ser43) antibody.
    Phosphospecificity is shown in the second lane (lambda-phosphatase: λ-Ptase).
    The blot is identical to the control except that the lysate was incubated in λ-Ptase (1400 units for 30 min).
    The immunolabeling is greatly decreased by treatment with λ-Ptase.