ARG10147

anti-Cardiac Troponin T antibody [1C11]

anti-Cardiac Troponin T antibody [1C11] for ELISA,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,IHC-Frozen sections,Immunohistochemistry (PFA perfusion fixed frozen sections),Immunoprecipitation and Dog,Human,Mouse,Rat

Cell Biology and Cellular Response antibody; Controls and Markers antibody; Developmental Biology antibody; Signaling Transduction antibody

Overview

Product Description

Mouse Monoclonal antibody [1C11] recognizes Cardiac Troponin T

Tested Reactivity Dog, Hu, Ms, Rat
Tested Application ELISA, ICC/IF, IHC-P, IHC-Fr, IHC-FoFr , IP
Specificity No cross-reaction with skeletal TnT.
Host Mouse
Clonality Monoclonal
Clone 1C11
Isotype IgG1
Target Name Cardiac Troponin T
Antigen Species Human
Immunogen free human cTnT
Conjugation Un-conjugated
Full Name troponin T type 2 (cardiac)
Alternate Names RCM3; CMPD2; LVNC6; TnTC; TnTc; CMH2; cTnT; Cardiac muscle troponin T; CMD1D; Troponin T, cardiac muscle

Application Instructions

Application Suggestion
Application Dilution
ELISAAssay-dependent
ICC/IFAssay-dependent
IHC-P1 µg/ml
IHC-FrAssay-dependent
IHC-FoFr Assay-dependent
IPAssay-dependent
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Protein A affinity purified.
Buffer PBS (pH 7.4) and 0.1% Sodium azide
Preservative 0.1% Sodium azide
Concentration 1.0-2.0 mg/ml
Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database links

GeneID: 21956 Mouse TNNT2

GeneID: 24837 Rat TNNT2

GeneID: 7139 Human TNNT2

Gene Symbol TNNT2
Background The protein encoded by this gene is the tropomyosin-binding subunit of the troponin complex, which is located on the thin filament of striated muscles and regulates muscle contraction in response to alterations in intracellular calcium ion concentration. Mutations in this gene have been associated with familial hypertrophic cardiomyopathy as well as with dilated cardiomyopathy. Transcripts for this gene undergo alternative splicing that results in many tissue-specific isoforms, however, the full-length nature of some of these variants has not yet been determined. [provided by RefSeq, Jul 2008]
Function Troponin T is the tropomyosin-binding subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity. [UniProt]
Resrarch Area Cell Biology and Cellular Response antibody; Controls and Markers antibody; Developmental Biology antibody; Signaling Transduction antibody
PTM Phosphorylation at Thr-213 by PRKCA induces significant reduction in myofilament calcium sensitivity and actomyosin ATPase activity.

Clone References

N-cadherin prevents the premature differentiation of anterior heart field progenitors in the pharyngeal mesodermal microenvironment.

IHC-Fr / Mouse

Soh BS et al.
Cell Res.,  (2014)

publication_link

 

hr_line

Superparamagnetic iron oxide nanoparticles function as a long-term, multi-modal imaging label for non-invasive tracking of implanted progenitor cells.

IHC-P / Rat

Pacak CA et al.
PLoS One.,  (2014)

publication_link

 

hr_line

RhoA/ROCK signaling and pleiotropic α1A-adrenergic receptor regulation of cardiac contractility.

WB / Mouse

Yu ZY et al.
PLoS One.,  (2014)

publication_link

 

hr_line

Analysis of the SWI/SNF chromatin-remodeling complex during early heart development and BAF250a repression cardiac gene transcription during P19 cell differentiation.

IHC-P / Mouse

Singh AP et al.
Nucleic Acids Res.,  (2014)

publication_link

 

hr_line

Fetal reprogramming and senescence in hypoplastic left heart syndrome and in human pluripotent stem cells during cardiac differentiation.

IHC, FACS / 

Gaber N et al.
Am J Pathol.,  (2013)

publication_link

 

hr_line

Pleiotropy of glycogen synthase kinase-3 inhibition by CHIR99021 promotes self-renewal of embryonic stem cells from refractory mouse strains.

ICC/IF / 

Ye S et al.
PLoS One.,  (2012)

publication_link

 

hr_line
Free antibody sample