ARG10451

anti-Cardiac Troponin T antibody [1F11]

anti-Cardiac Troponin T antibody [1F11] for ELISA,Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Bovine,Cat,Dog,Fish,Goat,Human,Mouse,Pig,Rabbit,Rat

Cell Biology and Cellular Response antibody; Controls and Markers antibody; Developmental Biology antibody; Signaling Transduction antibody

Overview

Product Description Mouse Monoclonal antibody [1F11] recognizes Cardiac Troponin T
Tested Reactivity Hu, Ms, Rat, Bov, Cat, Dog, Fsh, Goat, Pig, Rb
Tested Application ELISA, FACS, ICC/IF, IHC-P, WB
Specificity No cross-reaction with skeletal TnT.
Host Mouse
Clonality Monoclonal
Clone 1F11
Isotype IgG2b
Target Name Cardiac Troponin T
Antigen Species Human
Immunogen free human cTnT
Conjugation Un-conjugated
Full Name troponin T type 2 (cardiac)
Alternate Names RCM3; CMPD2; LVNC6; TnTC; TnTc; CMH2; cTnT; Cardiac muscle troponin T; CMD1D; Troponin T, cardiac muscle

Application Instructions

Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Protein A affinity purified.
Buffer PBS (pH 7.4) and 0.1% Sodium azide
Preservative 0.1% Sodium azide
Concentration 1.0-2.0 mg/ml
Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database links

GeneID: 21956 Mouse TNNT2

GeneID: 24837 Rat TNNT2

GeneID: 7139 Human TNNT2

Gene Symbol TNNT2
Gene Full Name troponin T type 2 (cardiac)
Background The protein encoded by this gene is the tropomyosin-binding subunit of the troponin complex, which is located on the thin filament of striated muscles and regulates muscle contraction in response to alterations in intracellular calcium ion concentration. Mutations in this gene have been associated with familial hypertrophic cardiomyopathy as well as with dilated cardiomyopathy. Transcripts for this gene undergo alternative splicing that results in many tissue-specific isoforms, however, the full-length nature of some of these variants has not yet been determined. [provided by RefSeq, Jul 2008]
Function Troponin T is the tropomyosin-binding subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity. [UniProt]
Research Area Cell Biology and Cellular Response antibody; Controls and Markers antibody; Developmental Biology antibody; Signaling Transduction antibody
PTM Phosphorylation at Thr-213 by PRKCA induces significant reduction in myofilament calcium sensitivity and actomyosin ATPase activity.

Clone References

Differential activation of natriuretic peptide receptors modulates cardiomyocyte proliferation during development.

Becker JR et al.
Development.,  (2014)

publication_link

 

hr_line

Zebularine regulates early stages of mESC differentiation: effect on cardiac commitment.

WB / Mouse

Horrillo A et al.
Cell Death Dis.,  (2013)

publication_link

 

hr_line

High density sphere culture of adult cardiac cells increases the levels of cardiac and progenitor markers and shows signs of vasculogenesis.

IHC / Human

Vukusic K et al.
Biomed Res Int.,  (2013)

publication_link

 

hr_line

Transplantation of parthenogenetic embryonic stem cells ameliorates cardiac dysfunction and remodelling after myocardial infarction.

IHC-P / Mouse

Liu Y et al.
Cardiovasc Res.,  (2013)

publication_link

 

hr_line

Conversion of mouse fibroblasts into cardiomyocytes using a direct reprogramming strategy.

FACS / Mouse

Efe JA et al.
Nat Cell Biol.,  (2011)

publication_link

 

hr_line

Conditional transgenic expression of fibroblast growth factor 9 in the adult mouse heart reduces heart failure mortality after myocardial infarction.

ICC/IF, IHC-Fr / Mouse

Korf-Klingebiel M et al.
Circulation.,  (2011)

publication_link

 

hr_line

Assaying cardiac biomarkers for toxicity testing using biosensing and cardiomyocytes derived from human embryonic stem cells.

IA / Human

Andersson H et al.
J Biotechnol.,  (2010)

publication_link

 

hr_line