anti-Cas9 (S. Pyogenes) antibody

anti-Cas9 (S. Pyogenes) antibody for ICC/IF,IHC-Frozen sections,Western blot and Bacteria


Product Description Rabbit Polyclonal antibody recognizes Cas9 (S. Pyogenes)
Tested Reactivity Bacteria
Tested Application ICC/IF, IHC-Fr, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name Cas9 (S. Pyogenes)
Antigen Species Bacteria
Immunogen N-terminal region, aa. 1-608 and C-terminal region, aa. 814-1372 of Cas9 sequence CDJ55032.1 from S. pyogenes, expressed in and purified from E. coli.
Conjugation Un-conjugated

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IF1:1000 - 1:2000
WB1:1000 - 1:10000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.


Form Liquid
Purification Affinity purification.
Buffer PBS and 50% Glycerol.
Stabilizer 50% Glycerol
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.


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Images (3) Click the Picture to Zoom In

  • ARG10734 anti-Cas9 (S. Pyogenes) antibody ICC/IF image

    Immunocytochemistry: Hek293 cell culture transfected with a construct including the C-terminal 814-1372 amino acids of Staphylococcus  pyogenes Cas9. The cells were stained with ARG10734 anti-Cas9 (S. Pyogenes) antibody (red) and also with a chicken antibody to S. pyogenes Cas9 (green). A cell expressing Cas9 therefore appears yellow. Most Hek293 cells are not transfected so only the nucleus of these cells can be visualized with the blue DNA stain.

  • ARG10734 anti-Cas9 (S. Pyogenes) antibody WB image

    Western blot: 1) 100 ng, and 2) 10 ng of full length Cas9 protein from Streptococcus pyogenes was stained with ARG10734 anti-Cas9 (S. Pyogenes) antibody at 1:2000 dilution. This antibody recognizes full length Cas9 protein at 160 kDa and a proteolytic fragment at 130 kDa.

  • ARG10734 anti-Cas9 (S. Pyogenes) antibody WB image

    Western blot: 1) HEK293 (non-transfected), 2) transfected HEK293 expressing the C-terminal aa. 814-1372 of Cas9 from S. pyogenes, 3) transfected HEK293 expressing the N-terminal 608 amino acids of Cas9 from S. pyogenes and 4) 40 ng of full length S. pyogenes Cas9 protein. The blots were stained with ARG10734 anti-Cas9 (S. Pyogenes) antibody (green) at 1: 1000 dilution.