anti-Cas9 (S. pyogenes) antibody [3F9]

anti-Cas9 (S. pyogenes) antibody [3F9] for ICC/IF,IHC-Frozen sections,Western blot and Bacteria


Product Description Mouse Monoclonal antibody [3F9] recognizes Cas9 (S. pyogenes)
Tested Reactivity Bacteria
Tested Application ICC/IF, IHC-Fr, WB
Host Mouse
Clonality Monoclonal
Clone 3F9
Isotype IgG
Target Name Cas9 (S. pyogenes)
Antigen Species Bacteria
Immunogen N-terminal region, aa. 1-608 of Cas9 sequence CDJ55032.1 from S. pyogenes, expressed in and purified from E. coli.
Conjugation Un-conjugated

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IF1:1000 - 1:2000
WB1:1000 - 1: 20000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.


Form Liquid
Purification Affinity purification.
Buffer PBS, 5 mM Sodium azide and 50% Glycerol.
Preservative 5 mM Sodium azide
Stabilizer 50% Glycerol
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.


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Images (2) Click the Picture to Zoom In

  • ARG10706 anti-Cas9 (S. pyogenes) antibody [3F9] ICC/IF image

    Immunocytochemistry: Transfected HEK293 cells overexpressing the N-terminal aa. 1-608 of S. pyogenes Cas9. The cells were stained with ARG10706 anti-Cas9 (S. pyogenes) antibody [3F9] (red), and these cells also co-stained with ARG10734 anti-Cas9 antibody to the same construct in green, giving a yellow color. The N-terminal construct contains a nuclear localization sequence and so is predominantly nuclear in localization. Most HEK293 cells in this field are not transfected so only the nuclei of these cells can be visualized with the blue DAPI DNA stain.

  • ARG10706 anti-Cas9 (S. pyogenes) antibody [3F9] WB image

    Western blot: 1) MW marker, 2) crude protein extract from Hek293 culture transfected with the immunogen, the N-terminal 1-608 amino acids, 3) Non-transfected control Hek293 cell extract, and 4) 40 ng of Streptococcus pyogenes Cas9 (full length) was stained with ARG10706 anti-Cas9 (S. pyogenes) antibody [3F9] at 1:1000 dilution.