anti-Caspase 1 antibody

anti-Caspase 1 antibody for Western blot and Mouse,Rat

Pyroptosis Study antibody; NLRP3 Inflammasome Study antibody; NLRC4 Inflammasome Study antibody
publication_link Publication1


Product Description Rabbit Polyclonal antibody recognizes Caspase 1
Tested Reactivity Ms, Rat
Tested Application WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name Caspase 1
Antigen Species Mouse
Immunogen A synthetic peptide corresponding to a sequence within the p20 subunit of mouse caspase 1 protein (137-157aa LEKAQKLWKENPSEIYPIMNT), the sequence is different from the rat caspase 1 protein sequence by three amino acids.
Conjugation Un-conjugated
Alternate Names Caspase-1; Interleukin-1 beta-converting enzyme; IL-1 beta-converting enzyme; CASP-1; ICE; IL-1BC; Interleukin-1 beta convertase; P45; IL1BC; p45; EC

Application Instructions

Application Suggestion
Tested Application Dilution
WB0.5 - 1 µg/ml
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.


Form Liquid
Purification Affinity purification with immunogen.
Buffer PBS, 0.025% Sodium azide and 2.5% BSA.
Preservative 0.025% Sodium azide
Stabilizer 2.5% BSA
Concentration 0.5 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.


Database Links

GeneID: 12362 Mouse CASP1

Swiss-port # P29452 Mouse Caspase-1

Gene Symbol Casp1
Gene Full Name caspase 1
Background Caspase 1 is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce 2 subunits, large and small, that dimerize to form the active enzyme. This gene was identified by its ability to proteolytically cleave and activate the inactive precursor of interleukin-1, a cytokine involved in the processes such as inflammation, septic shock, and wound healing. This gene has been shown to induce cell apoptosis and may function in various developmental stages. Studies of a similar gene in mouse suggest a role in the pathogenesis of Huntington disease. Alternative splicing results in transcript variants encoding distinct isoforms. [provided by RefSeq, Mar 2012]
Function Caspase 1: Thiol protease that cleaves IL-1 beta between an Asp and an Ala, releasing the mature cytokine which is involved in a variety of inflammatory processes. Important for defense against pathogens. Cleaves and activates sterol regulatory element binding proteins (SREBPs). Can also promote apoptosis. Upon inflammasome activation, during DNA virus infection but not RNA virus challenge, controls antiviral immunity through the cleavage of CGAS, rendering it inactive (PubMed:28314590). In apoptotic cells, cleaves SPHK2 which is released from cells and remains enzymatically active extracellularly (PubMed:20197547). [UniProt]
Cellular Localization Cytoplasm
Highlight Related products:
Caspase 1 antibodies; Caspase 1 ELISA Kits; Caspase 1 Duos / Panels; Anti-Rabbit IgG secondary antibodies;
Related news:
Exploring Antiviral Immune Response
RIP1 activation and pathogenesis of NASH
Research Area Pyroptosis Study antibody; NLRP3 Inflammasome Study antibody; NLRC4 Inflammasome Study antibody
Calculated MW 45 kDa
PTM The two subunits are derived from the precursor sequence by an autocatalytic mechanism.

Images (2) Click the Picture to Zoom In

  • ARG10644 anti-Caspase 1 antibody WB image

    Western blot: 20 μg of Raw264.7 cell lysate stained with ARG10644 anti-Caspase 1 antibody at 1:1000 dilution.

  • ARG10644 anti-Caspase 1 antibody WB image

    Western blot: 1) Rat brain, 2) Rat spleen, 3) Mouse brain, 4) Mouse spleen, and 5) Mouse testis stained with ARG10644 anti-Caspase 1 antibody.

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Sample Loading Amount:20 μg

Primary Antibody Dilution Factor:1:1000

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC

Specific References

Hydrogen gas inhalation attenuates sepsis-induced liver injury in a FUNDC1-dependent manner.

WB / Mouse

Yan Mengying et al.
Int Immunopharmacol.,  (2019)