ARG57293

anti-Caspase 1 antibody

anti-Caspase 1 antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation,Western blot and Human,Mouse

Pyroptosis Study antibody; NLRP3 Inflammasome Study antibody; NLRC4 Inflammasome Study antibody
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Overview

Product Description Rabbit Polyclonal antibody recognizes Caspase 1
Tested Reactivity Hu, Ms
Predict Reactivity Rat
Tested Application ICC/IF, IHC-P, IP, WB
Specificity This antibody reacts to full-length and cleaved form of caspase 1 protein.
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name Caspase 1
Antigen Species Human
Immunogen Recombinant Protein of Caspase 1.
Conjugation Un-conjugated
Alternate Names Caspase-1; Interleukin-1 beta-converting enzyme; IL-1 beta-converting enzyme; CASP-1; ICE; IL-1BC; Interleukin-1 beta convertase; P45; IL1BC; p45; EC 3.4.22.36

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IF1:25 - 1:200
IHC-P1:25 - 1:200
IP1:25 - 1:100
WB1:250 - 1:2000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Affinity purification with immunogen.
Buffer 1X PBS, 0.025% Sodium azide, 0.025% Thimerosal and 2.5% BSA
Preservative 0.025% Sodium azide and 0.025% Thimerosal
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 12362 Mouse CASP1

GeneID: 834 Human CASP1

Swiss-port # P29452 Mouse Caspase-1

Swiss-port # P29466 Human Caspase-1

Gene Symbol CASP1
Gene Full Name caspase 1, apoptosis-related cysteine peptidase
Background Caspase 1 is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce 2 subunits, large and small, that dimerize to form the active enzyme. This gene was identified by its ability to proteolytically cleave and activate the inactive precursor of interleukin-1, a cytokine involved in the processes such as inflammation, septic shock, and wound healing. This gene has been shown to induce cell apoptosis and may function in various developmental stages. Studies of a similar gene in mouse suggest a role in the pathogenesis of Huntington disease. Alternative splicing results in transcript variants encoding distinct isoforms. [provided by RefSeq, Mar 2012]
Function Caspase 1: Thiol protease that cleaves IL-1 beta between an Asp and an Ala, releasing the mature cytokine which is involved in a variety of inflammatory processes. Important for defense against pathogens. Cleaves and activates sterol regulatory element binding proteins (SREBPs). Can also promote apoptosis. Upon inflammasome activation, during DNA virus infection but not RNA virus challenge, controls antiviral immunity through the cleavage of CGAS, rendering it inactive (PubMed:28314590). In apoptotic cells, cleaves SPHK2 which is released from cells and remains enzymatically active extracellularly (PubMed:20197547). [UniProt]
Highlight Related Antibody Duos and Panels:
ARG30330 Pyroptosis Antibody Panel
ARG30331 NLRP3 Inflammasome Antibody Panel
ARG30332 NLRC4 Inflammasome Antibody Panel
Related products:
Caspase 1 antibodies; Caspase 1 ELISA Kits; Caspase 1 Duos / Panels; Anti-Rabbit IgG secondary antibodies;
Related news:
Exploring Antiviral Immune Response
RIP1 activation and pathogenesis of NASH
Research Area Pyroptosis Study antibody; NLRP3 Inflammasome Study antibody; NLRC4 Inflammasome Study antibody
Calculated MW 45 kDa

Images (5) Click the Picture to Zoom In

  • ARG57293 anti-Caspase 1 antibody WB image

    Western blot: 10 µg of HeLa and 20 µg of Mouse brain lysates stained with ARG57293 anti-Caspase 1 antibody at 1:1000 dilution.

  • ARG57293 anti-Caspase 1 antibody WB image

    Western blot: 25 µg of cell lysate from PMA treated THP-1 cells were stained with ARG57293 anti-Caspase 1 antibody at 1:1000 dilution.

  • ARG57293 anti-Caspase 1 antibody ICC/IF image

    Immunofluorescence: Raw264.7 cells stained with ARG57293 anti-Caspase 1 antibody at 1:100 dilution.

  • ARG57293 anti-Caspase 1 antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Mouse kidney tissue stained with ARG57293 anti-Caspase 1 antibody at 1:100 dilution.

  • ARG57293 anti-Caspase 1 antibody IP image

    Immunoprecipitation: 200 µg extracts of THP-1 cells Immunoprecipitated and stained with ARG57293 anti-Caspase 1 antibody at 1:1000 dilution.

Customer's Feedback

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Review for anti-Caspase 1 antibody

Application:WB

Sample:HeLa and Mouse brain

Sample Loading Amount:10 µg of HeLa and 20 µg of Mouse brain

Primary Antibody Dilution Factor:1:1000

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC

Specific References

Aronia Melanocarpa Polysaccharide Ameliotates Inflammation and Aging in Mice by Modulating AMPK/SIRT1/NF-κB Signaling Pathway and Gut Microbiota

WB / Mouse

Yingchun Zhao et al.
Preprint.,  (2021)

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