anti-Caspase 1 antibody
anti-Caspase 1 antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation,Western blot and Human,Mouse
Pyroptosis Study antibody; NLRP3 Inflammasome Study antibody; NLRC4 Inflammasome Study antibody
|Product Description||Rabbit Polyclonal antibody recognizes Caspase 1|
|Tested Reactivity||Hu, Ms|
|Tested Application||ICC/IF, IHC-P, IP, WB|
|Specificity||This antibody reacts to full-length and cleaved form of caspase 1 protein.|
|Target Name||Caspase 1|
|Immunogen||Recombinant Protein of Caspase 1.|
|Alternate Names||Caspase-1; Interleukin-1 beta-converting enzyme; IL-1 beta-converting enzyme; CASP-1; ICE; IL-1BC; Interleukin-1 beta convertase; P45; IL1BC; p45; EC 220.127.116.11|
|Application Note||* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.|
|Purification||Affinity purification with immunogen.|
|Buffer||1X PBS, 0.025% Sodium azide, 0.025% Thimerosal and 2.5% BSA|
|Preservative||0.025% Sodium azide and 0.025% Thimerosal|
|Storage Instruction||For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.|
|Note||For laboratory research only, not for drug, diagnostic or other use.|
|Gene Full Name||caspase 1, apoptosis-related cysteine peptidase|
|Background||Caspase 1 is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce 2 subunits, large and small, that dimerize to form the active enzyme. This gene was identified by its ability to proteolytically cleave and activate the inactive precursor of interleukin-1, a cytokine involved in the processes such as inflammation, septic shock, and wound healing. This gene has been shown to induce cell apoptosis and may function in various developmental stages. Studies of a similar gene in mouse suggest a role in the pathogenesis of Huntington disease. Alternative splicing results in transcript variants encoding distinct isoforms. [provided by RefSeq, Mar 2012]|
|Function||Caspase 1: Thiol protease that cleaves IL-1 beta between an Asp and an Ala, releasing the mature cytokine which is involved in a variety of inflammatory processes. Important for defense against pathogens. Cleaves and activates sterol regulatory element binding proteins (SREBPs). Can also promote apoptosis. Upon inflammasome activation, during DNA virus infection but not RNA virus challenge, controls antiviral immunity through the cleavage of CGAS, rendering it inactive (PubMed:28314590). In apoptotic cells, cleaves SPHK2 which is released from cells and remains enzymatically active extracellularly (PubMed:20197547). [UniProt]|
|Highlight||Related Antibody Duos and Panels:
ARG30330 Pyroptosis Antibody Panel
ARG30331 NLRP3 Inflammasome Antibody Panel
ARG30332 NLRC4 Inflammasome Antibody Panel
Caspase 1 antibodies; Caspase 1 ELISA Kits; Caspase 1 Duos / Panels; Anti-Rabbit IgG secondary antibodies;
Exploring Antiviral Immune Response
RIP1 activation and pathogenesis of NASH
|Research Area||Pyroptosis Study antibody; NLRP3 Inflammasome Study antibody; NLRC4 Inflammasome Study antibody|
|Calculated MW||45 kDa|
Images (5) Click the Picture to Zoom In
ARG57293 anti-Caspase 1 antibody WB image
Western blot: 10 µg of HeLa and 20 µg of Mouse brain lysates stained with ARG57293 anti-Caspase 1 antibody at 1:1000 dilution.
ARG57293 anti-Caspase 1 antibody WB image
Western blot: 25 µg of cell lysate from PMA treated THP-1 cells were stained with ARG57293 anti-Caspase 1 antibody at 1:1000 dilution.
ARG57293 anti-Caspase 1 antibody ICC/IF image
Immunofluorescence: Raw264.7 cells stained with ARG57293 anti-Caspase 1 antibody at 1:100 dilution.
ARG57293 anti-Caspase 1 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Mouse kidney tissue stained with ARG57293 anti-Caspase 1 antibody at 1:100 dilution.
ARG57293 anti-Caspase 1 antibody IP image
Immunoprecipitation: 200 µg extracts of THP-1 cells Immunoprecipitated and stained with ARG57293 anti-Caspase 1 antibody at 1:1000 dilution.