anti-Caspase 10 antibody

anti-Caspase 10 antibody for Western blot and Human

Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Metabolism antibody


Product Description Rabbit Polyclonal antibody recognizes Caspase 10
Tested Reactivity Hu
Tested Application WB
Specificity This antibody recognizes full-length human caspase-10 (59kDa). Since the sequences at C-termini of FLICE2 and Mch4 are different, this antibody recognizes only the FLICE2 form of caspase-10.
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name Caspase 10
Antigen Species Human
Immunogen Peptide corresponding to aa 505-521 at C-terminus of human FLICE2 (accession no. AAD28402).
Conjugation Un-conjugated
Alternate Names Caspase-10; EC; ICE-like apoptotic protease 4; Apoptotic protease Mch-4; FAS-associated death domain protein interleukin-1B-converting enzyme 2; FLICE2; CASP-10; ALPS2; MCH4

Application Instructions

Application Suggestion
Tested Application Dilution
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control HeLa, Jurkat, A431 and K562


Form Liquid
Purification Immunoaffinity chroma-tography
Buffer PBS (pH 7.4) and 0.02% Sodium azide
Preservative 0.02% Sodium azide
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.


Database Links

GeneID: 843 Human CASP10

Swiss-port # Q92851 Human Caspase-10

Gene Symbol CASP10
Gene Full Name caspase 10, apoptosis-related cysteine peptidase
Background A novel ICE/CED-3 protease was identified recently, designated FLICE2 and Mch4, and renamed as caspase-10. Caspase-10 has two death effector domains (DEDs) that bind to the DED in the adapter molecule FADD and recruits both TNFR1 and CD95 to form complexes with these receptors. Caspase-10 is, therefore, involved in CD95- and TNFR1-induced apoptosis. Caspase-10 cleaves and activates caspase-3, -4, -6, -7, -8, and -9, thereby causing proteolytic cleavage of many key proteins, such as PARP. Cleavage of PARP occurs in many different systems during apoptosis and is the hallmark of programmed cell death. Caspase-10 is expressed in many tissues and cell lines.
Function Involved in the activation cascade of caspases responsible for apoptosis execution. Recruited to both Fas- and TNFR-1 receptors in a FADD dependent manner. May participate in the granzyme B apoptotic pathways. Cleaves and activates caspase-3, -4, -6, -7, -8, and -9. Hydrolyzes the small- molecule substrates, Tyr-Val-Ala-Asp-|-AMC and Asp-Glu-Val-Asp-|-AMC.
Isoform C is proteolytically inactive. [UniProt]
Research Area Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Metabolism antibody
Calculated MW 59 kDa
PTM Cleavage by granzyme B and autocatalytic activity generate the two active subunits.

Images (1) Click the Picture to Zoom In

  • ARG54400 anti-Caspase 10 antibody WB image

    Western blot: H:HeLa; J:Jurkat; A:A431; K:K562 stained with ARG54400 anti-Caspase 10 antibody at 1 μg/ml dilution.