anti-Caspase 12 antibody

anti-Caspase 12 antibody for ELISA,Immunohistochemistry,Western blot,ICC/IF and Human,Mouse,Rat

Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Metabolism antibody; Neuroscience antibody
publication_link Publication5


Product Description Rabbit Polyclonal antibody recognizes Caspase 12
Tested Reactivity Hu, Ms, Rat
Tested Application ELISA, ICC/IF, IHC, WB
Specificity This antibody can be used for the detection of both the propeptide and cleaved forms of Caspase-12.
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name Caspase 12
Antigen Species Mouse
Immunogen Synthetic peptide (16 aa) within aa. 170-220 of Mouse Caspase-12.
Conjugation Un-conjugated
Alternate Names Inactive caspase-12; CASP-12; CASP12P1

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IF10 μg/ml
IHC2 μg/ml
WB2 μg/ml
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control Human Heart Tissue Lysate


Form Liquid
Purification Affinity purification with immunogen.
Buffer PBS and 0.02% Sodium azide
Preservative 0.02% Sodium azide
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.


Database Links

GeneID: 100506742 Human CASP12

GeneID: 12364 Mouse CASP12

GeneID: 156117 Rat CASP12

Gene Symbol Casp12
Gene Full Name caspase 12
Background Three distinct signaling pathways lead to programmed cell death (apoptosis). The death receptor and mitochondrion pathways are the mains, in which the key apoptotic proteases capase-8 and caspase-9, respectively, are involved. The endoplasmic reticulum (ER) stress is the third apoptotic pathway and caspase-12 is involved. Caspase-12 is localized to the ER but not to cytoplasm or mitochondrion. Caspase-12 is activated by ER stress, including disruption of ER calcium homeostasis, and mediates ER stress-induced apoptosis. Caspase-12 is co-localized to the ER with several proteins that are involved in Alzheimer's disease including gamma-secretase presenilin and beta-amyloid precursor protein (APP). Caspase-12 mediates cytotoxicity induced by amyloid-beta. Caspase-12 is ubiquitously expressed in mouse tissues.
Function Involved in the activation cascade of caspases responsible for apoptosis execution. [UniProt]
Highlight Related products:
Caspase12 antibodies; Anti-Rabbit IgG secondary antibodies;
Research Area Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Metabolism antibody; Neuroscience antibody
Calculated MW 39 kDa

Images (3) Click the Picture to Zoom In

  • ARG55177 anti-Caspase 12 antibody WB image

    Western blot: Human heart lysate stained with ARG55177 anti-Caspase 12 antibody at (A) 0.5, (B) 1, and (C) 2 ug/ml dilution.

  • ARG55177 anti-Caspase 12 antibody IHC image

    Immunohistochemistry: Human heart tissue stained with ARG55177 anti-Caspase 12 antibody at 2 ug/ml dilution.

  • ARG55177 anti-Caspase 12 antibody IHC image

    Immunohistochemistry: Caspase-12 in Human Heart tissue stained with ARG55177 anti-Caspase 12 antibody at 10 ug/ml dilution.

Customer's Feedback

nuts_pic      Excellent

Review for anti-Caspase 12 antibody


Sample:1) Mouse brain, 2) Rat brain

Sample Loading Amount:30 µg

Primary Antibody Dilution Factor:1:5000

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC

Specific References

17β-oestradiol alleviates endoplasmic reticulum stress injury induced by chronic cerebral hypoperfusion through the Haemoglobin/HIF 1α signalling pathway in ovariectomized rats

WB / Rat

Chao Xu et al.
Neurochem Int.,  (2021)




Down-regulation of calreticulin promotes apoptosis in hepatic stellate cells.

WB / Human

Linyu Dai et al.
第三军医大学学报,  (2021)




Interplay between Redox Signaling, Oxidative Stress, and Unfolded Protein Response (UPR) in Pathogenesis of Human Diseases.

WB / Rat

Poplawski Tomasz et al.
Oxid Med Cell Longev.,  (2019)




Hydrogen protects against chronic intermittent hypoxia induced renal dysfunction by promoting autophagy and alleviating apoptosis.

WB / Rat

Guan Peng et al.
Life Sci.,  (2019)




Hydrogen and Oxygen Mixture to Improve Cardiac Dysfunction and Myocardial Pathological Changes Induced by Intermittent Hypoxia in Rats.

WB / Rat

Zhao Ya-Shuo et al.
Oxidative Medicine and Cellular Longevity.,  (2019)