ARG54655

anti-Caspase 4 antibody

anti-Caspase 4 antibody for ELISA,Immunohistochemistry,Western blot,ICC/IF and Human,Mouse

Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Metabolism antibody

Overview

Product Description Rabbit Polyclonal antibody recognizes Caspase 4
Tested Reactivity Hu, Ms
Tested Application ELISA, ICC/IF, IHC, WB
Specificity Depending on cell lines or tissues used, other cleavage products may be observed.
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name Caspase 4
Immunogen Synthetic peptide (16 aa) within aa. 20-70 of Human Caspase-4.
Conjugation Un-conjugated
Alternate Names Mih1/TX; Protease ICH-2; Caspase-4; EC 3.4.22.57; TX; CASP-4; Protease TX; ICE; ICH-2; ICE(rel)II; rel; ICEREL-II

Application Instructions

Application Suggestion
Tested Application Dilution
ELISAAssay-Dependent
ICC/IF10 μg/mL
IHC2 μg/mL
WB0.5 - 1 μg/mL
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control Ramos Cell Lysate

Properties

Form Liquid
Purification Affinity purification with immunogen.
Buffer PBS and 0.02% Sodium azide
Preservative 0.02% Sodium azide
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 12363 Mouse CASP4

GeneID: 837 Human CASP4

Swiss-port # P49662 Human Caspase-4

Swiss-port # P70343 Mouse Caspase-4

Gene Symbol CASP4
Gene Full Name caspase 4, apoptosis-related cysteine peptidase
Background Caspase-4 Antibody: Caspases are a family of cysteine proteases that can be divided into the apoptotic and inflammatory caspase subfamilies. Unlike the apoptotic caspases, members of the inflammatory subfamily are generally not involved in cell death but are associated with the immune response to microbial pathogens. Members of this subfamily include caspase-1, -4, -5, and -12. Activation of these caspases results in the cleavage and activation of proinflammatory cytokines such as IL-1β and IL-18. Caspase-4 was initially identified as a homologous protein to Caspase-1 and the C. elegans Ced-3 which could induce apoptosis in transfected cells. More recent studies have shown that it can be activated by ER stress and has been suggested to be involved in multiple neuronal pathologies such as Alzheimer's disease. |
Research Area Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Metabolism antibody
Calculated MW 43 kDa
PTM In response to activation signals, including endoplasmic reticulum stress or treatment with amyloid beta A4 protein fragments (such as beta-amyloid protein 40), undergoes autoproteolytic cleavage.

Images (3) Click the Picture to Zoom In

  • ARG54655 anti-Caspase 4 antibody ICC/IF image

    Immunofluorescence: Mouse spleen 100 cells stained with ARG54655 anti-Caspase 4 antibody at 10 μg/ml.

  • ARG54655 anti-Caspase 4 antibody IHC image

    Immunohistochemistry: Mouse spleen stained with ARG54655 anti-Caspase 4 antibody at 2 μg/ml.

  • ARG54655 anti-Caspase 4 antibody WB image

    Western blot: Ramos cells stained with ARG54655 anti-Caspase 4 antibody at (A) 0.5 and (B) 1 μg/ml.